In an accompanying paper (l), it is shown that y-(p-arsenosophenyl)-n-butyrate activates the adenosine triphosphatase of both myosin and mitochondria, and several analogies between the properties of the active sites of these two energy transduction enzymes are discussed. Carbonyl cyanide p-chlorophenylhydrazone, which uncouples osidative phosphorylation at concentrations as low as lop7 &r (a), ...

1. Treatment of liver microsomal fraction with 0.03-0.12% sodium deoxycholate and 0.005-0.06 mM carbonyl cyanide m-chlorophenylhydrazone decreases phospholipid-dependent hydrophobicity of the microsomal membrane, assayed by the kinetics of 8-anilinonaphthalene-1-sulphonate binding and ethyl isocyanide difference spectra. 2. Sodium deoxycholate at a concentration of 0.01% lacks its detergent pro...

background: acinetobacter baumannii is an important human pathogen with increasing notoriety in the recent years, as a causative organism of drug resistant nosocomial infections, particularly in immunocompromised patients hospitalized in burn centers. objectives: the aim of this study was to determinate the role of efflux pump(s) in ciprofloxacin resistance of a. baumannii strains isolated from...

To test the influence of the electrochemical ion gradient across mycoplasma membranes on the capacity of organisms to adhere to host cells, Mycoplasma gallisepticum cells were treated with valinomycin, carbonylcyanide m-chlorophenylhydrazone, and N,N'-dicyclohexylcarbodiimide (DCCD) singly or in combination. Uptake of [3H]tetraphenylphosphonium by the treated cells was employed as a measure of ...

The transport of precursor proteins into mitochondria requires an energized inner membrane. We report here that the import of various precursor proteins showed a differential sensitivity to treatment of the mitochondria with the uncoupler carbonyl cyanide m-chlorophenylhydrazone. The differential inhibition by carbonyl cyanide m-chlorophenylhydrazone was not influenced by the length of the prec...

Cytochrome c oxidase binds protonophore carbonyl cyanide 3-chlorophenylhydrazone (CCCP) with high affinity. There are 1.46 high-affinity binding sites per cytochrome c oxidase for CCCP with dissociation constant 2.7 x 10(-7) mol/l. The bond between the CCCP and cytochrome c oxidase accomplishes through the group on cytochrome c oxidase with pKa 6.64 and is based on the electrostatic interaction...

Laguna, Tenerife, Canarias, Spain haemand Mo-dependent and Mo-dependent activities of nitrate reductase, determined with the non-physiological electron donors FMNH, and reduced methyl viologen respectively, were less affected. A similar inactivation was found with the proton ionophores 2,rddinitrophenol and carbonyl cyanide m-chlorophenylhydrazone. The inactive enzyme was found in the particula...