Adult East Freisan crossbred (n = 220) and Chios ewes (n = 105) were divided into four groups and inseminated with chilled semen, which had been stored for 7, 13, 20 or 26 h at 5°C. Unilateral intrauterine insemination (50 x 10 6 spermatozoa in 0.25 ml) was performed with the aid of a laparoscope. Inseminations were carried out 48 – 52 h after pessary removal (30 mg FGA) without detecting estru...

AIM The objective of this study was to evaluate the effects of different concentrations of soybean lecithin (SL) and virgin coconut oil (VCO) in Tris-based extender on chilled and frozen-thawed bull semen quality parameters. MATERIALS AND METHODS A total of 24 ejaculates were collected from four bulls via an electroejaculator. Semen samples were diluted with 2% VCO in Tris-based extender whic...

The technique (sperm chromatin structure assay) used in this study identifies one reason (a decrease in chromatin quality) for stallions whose semen shows reduced fertility when chilled. This assay is a diagnostic test, in addition to the use of sperm motility and morphologic evaluation, to evaluate the quality of chilled stallion semen and further aid the practitioner and the breeding industry...

BACKGROUND Artificial insemination (AI) using frozen-thawed semen is well established and routinely used for breeding in various mammalian species. However, there is no report of the birth of elephant calves following AI with frozen-thawed semen. The objective of the present study was to investigate the fertilizing ability of chilled and frozen-thawed semen in the Asian elephant following artif...

Artificial insemination (AI) is becoming more common in canine reproduction, permitting the use of shipped semen, assistance for geriatric or subfertile males, coverage of dominant females, and advanced reproductive technology such as intrauterine deposition of semen. AI may be performed with fresh, chilled, or frozen semen. All instruments should be clean and free of any chemical contamination...

This article covers recent developments in the field of equine artificial insemination. Use of both chilled and frozen semen is discussed. For chilled semen, there is new information about what to do if the semen shipment is delayed and on maximising pregnancy rates in older mares by careful evaluation of the cervix. The number of motile sperm needed for insemination is reviewed in light of the...

the objective of the present study was to examine the effects of chilling period on semen quality of sannan and jamunapari. semen of goat breeds were collected with artificial vagina and chilled at 5-9˚c. volume, colour and ph were determined on fresh semen. after dilution, microscopic examination was performed for the progressive motility and sperm viability. diluted semen of both breed was co...

The objective of the present study was to examine the effects of chilling period on semen quality of Sannan and Jamunapari. Semen of goat breeds were collected with artificial vagina and chilled at 5-9˚C. Volume, colour and pH were determined on fresh semen. After dilution, microscopic examination was performed for the progressive motility and sperm viability. Diluted semen of both breed was co...

Background: The application of laparoscopic method for intrauterine insemination is limited in sheep industry by the high cost of equipment and low pregnancy rate of frozen-thawed spermatozoa. Both disadvantages could be decreased if chilled ram semen is transported to the farms and inseminated through cervix. Semen extender provides the requirements for sperm and affects its motility, viabilit...

Although dog was the first species to be used for Artificial Insemination (AI) and become pregnant by Spallanzani in 1776, very little work has been done on preservation of dog semen to exploit the full genetic potential of pedigree males. Frozen semen is now inseminated routinely in cattle, sheep and goats and much research has been carried out with regard to the optimum conditions for its use...