An intracellular protease was extracted and purified from Pseudomonas aeruginosa by ion-exchange chromatography on DEAE-cellulose followed by CM–cellulose and rechromatography on DEAE-cellulose. The purified protease was found to be homogeneous as judged by polyacrylamide disc gel electrophoresis (PAGE). The molecular mass of the protease as determined by gel filtration on G-150 was about 48,00...

In order to develop novel polymeric supports based on cellulosics, cellulose extracted from pine needles, a perennial resource material available in huge quantities as waste, was graft copolymerized with vinyl monomers. Cellulose, cellulose derivatives, and their graft copolymers with highest percent grafting (Pg) were used as supports for immobilization of an industrially important protease en...

A novel protease with a molecular mass of 15 kDa was purified from fresh fruiting bodies of the wild mushroom Amanita farinosa. The purification protocol entailed anion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, cation exchange chromatography on SP-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. The protease was u...

A new protease was found in mitochondria of bone marrow cells. The protease was purified from human bone marrow cells by using the following methods: disruption of cells by sonication; buffer extraction; column chromatography using DEAE-cellulose, Sephadex G-75, and CM-cellulose. It was then crystallized in the presence of polyethylene glycol. The crystallized enzyme is homogeneous as judged by...

This article reports the ginger protease extraction and purification methods from fresh ginger rhizome. As to ginger protease extraction, we adapt the steps of organic solvent dissolving, ammonium sulfate depositing and freeze-drying, and this method can attain crude enzyme powder 0.6% weight of fresh ginger rhizome. The purification part in this study includes two steps: cellulose ion exchange...

A procedure using DEAE cellulose and hydroxylapetite was developed for the separation of Aeromonas salmonicida P1 protease, P2 protease, and trout cell-specific hemolysin (T-lysin) from supernatants of broth cultures. The Mferent proteases were demonstrated using protease inhibitors, substrate specificities, and polyacrylamide gel electrophoresis with protease substrates in the gels. Isolated P...

A novel aspartic protease with HIV-1 RT inhibitory activity was isolated and characterized from fruiting bodies of the wild mushroom Xylaria hypoxylon. The purification protocol comprised distilled water homogenization and extraction step, three ion exchange chromatographic steps (on DEAE-cellulose, Q-Sepharose, and CM-cellulose in succession), and final purification was by FPLC on Superdex 75....

Isolation of endogenous proteins from Saccharomyces cerevisiae has been facilitated by inserting encoding polypeptide affinity tags at the C-termini of chromosomal open reading frames (ORFs) using homologous recombination of DNA fragments. Tagged protein isolation is limited by a number of factors, including high cost of affinity resins for bulk isolation and low concentration of ligands on the...

Protease inhibitors were extracted from the seeds of Lavatera cashmeriana Camb by ammonium sulphate precipitation and purified by chromatography on DEAE-cellulose and Sephadex G-100 column. The bound protein eluted into four major peaks which we named as LC-pi I, II, III and IV, all showing strong anti protease activity against trypsin, chymotrypsin and elastase. LC-pi I was screened for antiba...