the pharmacokinetics of recombinant human erythropoietin (rhuepo) administered intravenously has been investigated in a group of five patients with chronic renal failure who were dialysis-dependent. the half-life of circulating erythropoietin decreased from 7.9±oa hr (mean ±so) at the beginning of treatment to 6.2±o.6 hr after 6 weeks and 5a±o.9 hr after 4 months of treatment. in spite of the s...

nowadays nanotechnology as a new direction of science allows us to develop therapeutic methods of the endogenous intoxication syndrome and to create a new class of biocompatible sorbents. in ukraine first preparations of medical nanotechnology were produced and patented in 1998. these are ikbb (intracorporeal biocorrector), magnet-controlled sorbent (mcs-b), and micromage-b. the preparations ar...

Objective(s): Chronic myelogenous leukemia (CML) is a chronic myeloproliferative disorder characterized by the accumulation of myeloid cells with a chromosomal translocation known as the Philadelphia chromosome. In this study, we investigated the roles of miR-570 in CML development. Materials and Methods: Expression of miR-570 in CML samples and cell lines was determined by qRT-PCR. Glucose upt...

ceramide as a second messenger is a key regulator in apoptosis and cytotoxicity. ceramide-metabolizing enzymes are ideal target in cancer chemo-preventive studies. neutral sphingomyelinase (nsmase), acid ceramidase (acdase) and glucosyl ceramide synthase (gcs) are the main enzymes in ceramide metabolism. silymarin flavonolignans are potent apoptosis inducers and silibinin is the most active com...

Ceramide as a second messenger is a key regulator in apoptosis and cytotoxicity. Ceramide-metabolizing enzymes are ideal target in cancer chemo-preventive studies. Neutral sphingomyelinase (NSMase), acid ceramidase (ACDase) and glucosyl ceramide synthase (GCS) are the main enzymes in ceramide metabolism. Silymarin flavonolignans are potent apoptosis inducers and silibinin is the most active com...

Ceramide as a second messenger is a key regulator in apoptosis and cytotoxicity. Ceramide-metabolizing enzymes are ideal target in cancer chemo-preventive studies. Neutral sphingomyelinase (NSMase), acid ceramidase (ACDase) and glucosyl ceramide synthase (GCS) are the main enzymes in ceramide metabolism. Silymarin flavonolignans are potent apoptosis inducers and silibinin is the most active com...

Catechin, epicatechin gallate (ECG) and quercetin, as bioactive flavonoids, have been shown to possess anticarcinogenic effects. Ceramide plays an important role in killing tumor cells. Accordingly, the aim of this study was to clarify the involvement of these compounds in ceramide metabolism in A549 cancerous cell line. Spectrophotometer, cell culture and HPLC methods were used. Cell viability...

one of the main problems in cell culture is mycoplasma infection. it can extensively affect cell physiology and metabolism. as the applications of cell culture increase in research, industrial production and cell therapy, more concerns about mycoplasma contamination and detection will arise. this review will provide valuable information about: 1. the ways in which cells are contaminated and the...

When plants encounter external environmental stimuli, they can adapt to changes through a complex network of metabolism–gene expression–metabolism within the plant cell. In this process, in characteristics cells are phenotype that is responsive and directly linked network. Accurate identification large numbers quantitative analysis their cellular much-needed experiment for in-depth metabolism g...