Our previous studies revealed an inhibitory effect of ICD-85 (Venom derived peptides) on breast cancer cell line MDA-MB231. ICD-85 was also confirmed by in-vivo studies to suppress the breast tumor in mice. However, the exact mechanism of ICD-85 was unknown. Hence, the present study was undertaken to assess the mechanism of ICD-85 effect as an anti-proliferative agent of cancer cells. The effec...

Our previous studies revealed an inhibitory effect of ICD-85 (Venom derived peptides) on breast cancer cell line MDA-MB231. ICD-85 was also confirmed by in-vivo studies to suppress the breast tumor in mice. However, the exact mechanism of ICD-85 was unknown. Hence, the present study was undertaken to assess the mechanism of ICD-85 effect as an anti-proliferative agent of cancer cells. The effec...

Proteinase inhibitor I has been identified and quantified in isolated vacuoles from tomato (Lycopersicon esculentum) leaves induced to accumulate inhibitors either by wounding or by supplying excised leaves with the wound hormone, proteinase inhibitor-inducing factor. Proteinase inhibitor II was also identified in the vacuoles but not quantified. Control vacuoles were prepared from unwounded pl...

our previous studies revealed an inhibitory effect of icd-85 (venom derived peptides) on breast cancer cell line mda-mb231. icd-85 was also confirmed by in-vivo studies to suppress the breast tumor in mice. however, the exact mechanism of icd-85 was unknown. hence, the present study was undertaken to assess the mechanism of icd-85 effect as an anti-proliferative agent of cancer cells. the effec...

Macrophages from rabbit peritoneal exudate cells incubated in vitro with T(2) bacteriophage from 10 up to 120 minutes show phage particles adsorbed to cell membranes, in the process of being engulfed by means of rhopheocytosis, micropinocytosis, and phagocytosis, and localized within dense vacuoles, semi-dense vacuoles, and clear vacuoles of the cytoplasm. The electronmicrographs suggest that n...

Mouse peritoneal macrophages have been studied in vitro after ingestion of treated rat, rabbit, or sheep erythrocytes. Under light microscopy, phagocytic vacuoles persist up to 24 h. Macrophages lose benzidine reactivity about 5 h after red cell ingestion, and they become prussian blue positive at 2 days. Ultrastructural studies show little or no ferritin in control macrophages not fed erythroc...

Ultrastructural changes during encystation of Polysphondylium palltdum, a cellular slime mould, include an increase in fibnllar material in the cytoplasmic matrix, the formation of cytoplasms microprojections at the cell periphery and the occurrence of tiny vesicles and some larger vacuoles near the cell periphery. The cyst wall appears first as a fluffy, loose network of fibrils. In the mature...

The development of techniques to isolate and purify relatively large quantities of intact vacuoles from mature tissues permits direct biochemical analysis of this ubiquitous mature plant cell organelle. Vacuoles and a fraction enriched in soluble cytoplasmic constituents were quantitatively prepared from Hippeastrum flower petal protoplasts. Vacuolar lysate and soluble cytoplasmic fractions wer...

in two of the three sublines virus-like particles of type C were present within cytoplasmic vacuoles and outside the cells close to the cell surface. The particles formed by a budding process at the ceil membrane. Similar particles were observed in pellets prepared from the medium in which the cells had been grown, and in cells of tumors produced in C3H mice by inoculation of the cultured cells...