mycoplasma and ureaplasma species are the most serious contaminants of cell cultures and this remains one of the major problem encountered in biological research using cell cultures. the aim of this study was to investigate the rate of mycoplasma and a total of 100 different cell culture specimens including, r228, hela, vero, mdck, l20, rd and hep2 were collected. then the specimens were invest...

background and objectives : each year, enteroviruses infect millions of people and cause different diseases. the agents are usually detected using cell culture. rd (rhabdomyosarcoma) and l20b (l cells) are among the recommended cells by the world health organisation (who) for this purpose. even though cell culture is the most common method used in diagnosing enteroviruses in stool specimens, th...

the enzymatic potential of the cultured plant cells can be employed for bioconversion purposes. plant enzymes are able to catalyze regio- and stereo-specific reactions, and therefore can be applied for the production of desired substances. the biotransformation of foreign substrates with suspension cells of peganum harmala was tested with (±) phenylethyl propionate. the callus cultures of pegan...

There are some reports on the lignan constituents of Linum spp. In this study, we investigated the cyototoxicity of Linum spp. in vitro cultures for the first time. If they show significant cytotoxic activity, they will be valuable candidates for isolation of cytotoxic compounds in large scale from them by tissue culture technology. In our study, we chose normal root, hairy root and calli cultu...

cell cultures of varthemia persica, peganum harmala and pycnocycla spinosa have been studied to evaluate their abilities to bioconvert exogenous hydroquinone. arbutin is an important substance that has several pharmaceutical applications; therefore, we have established v. persica and p. spinosa cultures which seem to be able to metabolize hydroquinone. callus cultures of v. persica were establi...

There are some reports on the lignan constituents of Linum spp. In this study, we investigated the cyototoxicity of Linum spp. in vitro cultures for the first time. If they show significant cytotoxic activity, they will be valuable candidates for isolation of cytotoxic compounds in large scale from them by tissue culture technology. In our study, we chose normal root, hairy root and calli cultu...

Zinc and iron nano-oxides (100 ppb) were promoted the hypericin and hyperforin production in Hypericum perforatum cell suspension culture. High performance liquid chromatography method was used for detectection and identification of hypericin and hyperforin in H. perfuratum cell suspension cultures elicited with different concentrations of zinc and iron nano-oxide (0, 50, 100 and 150 ppb) after...

This paper reviews recent strategies used for increasing specific yield and productivity in high cell density cultures. High cell density cultures offer an efficient means for the economical production of recombinant proteins. However, there are still some challenges associated with high cell density cultivation (HCDC) techniques. A variety of strategies in several aspects including host design...

Background: Plumbagin is as an important bioactive secondary metabolite found in the roots of Plumbago spp. The only one species, Plumbago europaea L., grows wild in Iran. The therapeutic use of plumbagin is limited due to its insufficient supply from the natural sources as the plants grow slowly and take several years to produce quality roots. O...