cystic hydatid disease (hydatidosis) is the most serious tape-worm infection prevalent in the cattle and sheep raising area of the world. hydatidosis in man (as an accidental host) caused by infection with the ova containing larval stage of echinococcus spp. in the last decade different techniques have been employed for the serological diagnosis of hydatid disease, such as iha, ifa, elisa, ccle...

early diagnosis of human hydatid disease by detecting the specific antibodies in patients’ sera is considered as an important step in treatment of infection. but the diagnostic efficiencies of assays greatly depend on the characteristics of antigen that is used and various conditions in performance. in present study, we tried to standardize an indirect haemagglutination test, using antigen b fo...

A rapid, simple, and accurate Modified Counter Current Immuno-electrophoresis (MCCIE) technique was developed and compared with the Reverse Passive Heamagglutination Assay (RPHA) for the titration of HPS virus. The MCCIE test had 100% correlation with the conventional RPHA and correctly titrated 116 samples which were already titrated with RPHA. Instead of horizontal electrophoresis chamber use...

A total of 231 serum samples were collected from sheep (n=9), goats (n=99) and cattle (n=123) in northeastern KwaZulu-Natal, South Africa. Trypanosome infection was detected using Trypanosoma brucei brucei crude antigen (TbbCA) and T. congolense crude antigen (TcoCA) ELISA assays. Recombinant antigen (T. evansi GM6 which consisted of 4 repeat domains, TeGM6-4r) ELISA and immunochromatographic t...

background: echinococcosis is a parasitic disease with worldwide distribution which is caused by the tapeworms echinococcus granulosus . diagnosis of the disease relies on imaging techniques, but the techniques are not able to differentiate the cyst from benign or malignant tumors; hence, appropriate serologic methods are required for the differential diagnosis of the infection. materials and m...

tropical fasciolosis caused by infection with fasciola gigantica is regarded as one of the most important single helminthes infections of ruminants in asia and africa. fasciolosis diagnosis, due to low sensitivity of coprological diagnostic method has been challenging for a long period. to evaluate the usefulness of elisa for diagnosis of anti – fasciola gigantica antibodies in parasitized shee...

Simian virus 40 T antigen binds to both single and double strand DNA. The single and double strand DNA binding activity of crude T antigen preparations was evaluated by chromatography of the antigen on DNA-cellulose columns. Crude T antigen was retained on both native and denatured DNA-cellulos columns and was eluted from both columns under similar conditions. The interaction of highly purified...

in this study, a crude leishmanial antigen, was prepared by sonicating leishmania major promastigotes used to induce immunity in balb/c mice against cutaneous leishmaniasis. correlation between the route of antigen injection and the efficacy of induced protection was examined. to enhance the effectiveness of the antigen, an immunostimulant drug, daraprim (pyrimethamine), was administered simult...

Background: Hydatidosis is one of the cosmopolitan parasitic zoonoses caused by the larval stage of Echinococcus granulosus. Diagnosis of hydatidosis is still an unresolved problem. Serological tests using crude antigens for diagnosis of E. granulosus are sensi-tive, however their specificity are not satisfactory. Therefore, WHO recommended spe-cific serological methods using specific antigens,...