Chronic bee paralysis virus (CBPV) causes an infectious and contagious disease of adult honeybees. Its segmented genome is composed of two major positive single-stranded RNAs, RNA 1 (3,674 nt) and RNA 2 (2,305 nt). Three minor RNAs (about 1,000 nt each) have been described earlier but they were not detected by sequencing of CBPV genome. In this study, the results of in vivo inoculation of the t...

A two-step real-time RT-PCR assay, based on TaqMan technology using a fluorescent probe (FAM-TAMRA) was developed to quantify Chronic bee paralysis virus (CBPV) genome in bee samples. Standard curves obtained from a CBPV control RNA and from a plasmid containing a partial sequence of CBPV showed that this assay provided linear detection over a 7-log range (R(2)>0.99) with a limit of detection o...

The call-by-push-value (CBPV) calculus is a general framework within which one can study computational effects such as state, nondeterminism, and input/output. Compared to the simply typed lambda calculus, the CBPV type system is much more finegrained and distinguishes between value and computation types. We give a self-contained proof of strong normalization for CBPV, employing a notion of >>-...

Chronic bee paralysis virus (CBPV) is an important viral disease of adult bees which induces significant losses in honey bee colonies. Despite comprehensive research, only limited data is available from experimental infection for this virus. In the present study winter worker bees were experimentally infected in three different experiments. Bees were first inoculated per os (p/o) or per cuticle...

Chronic bee paralysis virus (CBPV) causes an infectious and contagious disease of adult honeybees. Here, we report the complete genome sequence of CBPV strain SLO/M92/2010. This is the first published complete genome of CBPV in Apis mellifera carnica, which provides important additional knowledge about the divergence of the CBPV genome.

We present the call-by-push-value (CBPV) calculus, which decomposes the typed call-by-value (CBV) and typed call-by-name (CBN) paradigms into fine-grain primitives. On the operational side, we give big-step semantics and a stack machine for CBPV, which leads to a straightforward push/pop reading of CBPV programs. On the denotational side, we model CBPV using cpos and, more generally, using alge...

Chronic bee paralysis virus (CBPV) is a serious viral disease affecting adult bees. We report here the complete genome sequence of CBPV, which was isolated from a honey bee colony with the symptom of severe crawling. The genome of CBPV consists of two segments, RNA 1 and RNA 2, containing respective overlapping fragments.

Chronic bee paralysis virus (CBPV) infection causes chronic paralysis and loss of workers in honey bee colonies around the world. Although CBPV shows a worldwide distribution, it had not been molecularly detected in Japan. Our investigation of Apis mellifera and Apis cerana japonica colonies with RT-PCR has revealed CBPV infection in A. mellifera but not A. c. japonica colonies in Japan. The pr...

BACKGROUND Cardiovascular disease (CVD) risk, although perceived to be high, is often difficult to demonstrate in disease free (healthy) obese adults. HYPOTHESIS Changes in circadian blood pressure variability (CBPV) and endothelial function (EF) may be early correlates of cardiometabolic disorders. METHODS Asymptomatic men and women in 3 groups: normal weight (n = 10), overweight (n = 10) ...

Chronic bee paralysis virus (CBPV) is responsible for chronic paralysis, an infectious and contagious disease of adult honey bees (Apis mellifera L.). The full-length nucleotide sequences of the two major RNAs of CBPV have previously been characterized. The Orf3 of RNA1 has shown significant similarities to the RNA-dependent RNA polymerase (RdRp) of positive single-stranded RNA viruses, whereas...