Barley powdery mildew, caused by the pathogen Blumeria graminis f. sp. hordei is an important disease of barley (Hordeum vulgare L.). The random amplified polymorphic DNA (RAPD) method was used to detect DNA polymorphism among 7 Pallas near-isogenic lines (NILs) carrying Mla3, Mla12, Mlk, Mlp, Mlat, Mlg and MlLa genes for resistance to B. graminis f. sp. hordei. From among 500 random 10-mer pri...

Barley ( Hordeum vulgare ) is an important crop in Turkey. In this study, 37 barley fields Batman central district and Beşiri (Batman), Gercüş Hasankeyf Kozluk Sason Kocaköy (Diyarbakır), Kurtalan (Siirt), Midyat (Mardin) districts were examined for the presence of leaf diseases. addition, 24 naturally grown wild H. spontaneum populations Beşiri, Hasankeyf, inspected fields, scald caused by Rhy...

Plant processes resulting from primary or secondary metabolism have been hypothesized to contribute to defense against microbial attack. Barley chorismate synthase (HvCS), anthranilate synthase alpha subunit 2 (HvASa2), and chorismate mutase 1 (HvCM1) occupy pivotal branch points downstream of the shikimate pathway leading to the synthesis of aromatic amino acids. Here, we provide functional ev...

Obligate biotrophic fungal pathogens, such as Blumeria graminis and Puccinia graminis, are amongst the most devastating plant causing dramatic yield losses in many economically important crops worldwide. However, a lack of reliable tools for efficient genetic transformation has hampered studies into molecular basis their virulence or pathogenicity. In this study, we present Ustilago hordei–barl...

Reactive oxygen intermediates (ROI) are closely related to defence reactions of plants against pathogens. A prominent role in the production of ROI has been attributed to the plant respiratory burst oxidase homologues (RBOH) of the human phagocyte GP91(phox). A barley RBOH, which encodes a putative superoxide (O2*-)) producing NADPH oxidase, is described here. Histochemical analysis of the barl...

To study protein ubiquitination pathways in the interaction of barley (Hordeum vulgare) with the powdery mildew fungus (Blumeria graminis), we measured protein turnover and performed transient-induced gene silencing (TIGS) of ubiquitin and 26S proteasome subunit encoding genes in epidermal cells. Attack by B. graminis hyperdestabilized a novel unstable green fluorescent protein fusion that cont...

The previously compiled list of species powdery mildew fungi Ukraine was published more than 30 years ago. That included 108 belonging to 12 genera. However, over the past three decades, numerous changes have been made in system former order Erysiphales, particular, its generic composition; moreover, dozens new described. At same time, many were introduced Europe, including Ukraine, from East A...

Barley (Hordeumvulgare L.) is one of the most important cereal crops in world. Powdery mildew on barley, which caused by pathogen Blumeria graminis f. sp. hordei, occurs world-wide and can result severe yield loss. Thousands barley accessions are stored national gene banks, their characterization for breeding purposes needed. This study was conducted to determine resistance powdery 33 landraces...

Many fungal pathogens must enter plant cells for successful colonization. Barley mildew resistance locus o (Mlo) is required for host cell invasion upon attack by the ascomycete powdery mildew fungus, Blumeria graminis f.sp. hordei, and encodes the founder of a family of heptahelical integral membrane proteins unique to plants. Recessively inherited loss-of-function mutant alleles (mlo) result ...