objective the extensive pollution of natural ecosystems has occurred by organophosphorus pesticides, being used for improvement of crop production in agriculture. biodegradation is interested in application of multifunctional bacteria containing genetical elements which specially degrade organophosphorus compounds. in this paper the detection of two bacteria species, pseudomonas and flavobacter...

Classical enzyme kinetic theories are summarized and linked with modern discoveries here. The sequential catalytic events along time axis by analyzed at the molecular level, using master equations, this writing tries to connect microscopic behavior of data (like velocity coefficient k) obtained in experiment: 1/k = t equals sum times taken constituent individual steps. relationships between k, ...

Currently there is a significant amount of information about the way biocatalysts from animals detoxicate and bioactive drugs of abuse. In some cases, biotransformation data concerning drugs of abuse obtained from animal systems are analogous to the human situation, but in many cases the data are not. Clearly, significant work needs to be done with human biocatalysts to define a role in the bio...

Multi-site enzymes, defined as where multiple substrate molecules can bind simultaneously to the same enzyme molecule, play a key role in a number of biological networks, with the Escherichia coli protease ClpXP a well-studied example. These enzymes can form a low latency 'waiting line' of substrate to the enzyme's catalytic core, such that the enzyme molecule can continue to collect substrate ...

The EzCatDB (Enzyme Catalytic-mechanism Database) specifically includes catalytic mechanisms of enzymes in terms of sequences and tertiary structures of enzymes, and proposed catalytic mechanisms, along with ligand structures. The EzCatDB groups enzyme data in the Protein Data Bank (PDB) and the SWISS-PROT database with identical domain compositions, Enzyme Commission (EC) numbers and catalytic...

15 Much of the functional diversity observed in modern enzyme superfamilies originates from 16 molecular tinkering with existing enzymes. New enzymes frequently evolve from enzymes 17 with latent, promiscuous activities, and often inherit key features of the ancestral enzyme, 18 retaining conserved catalytic groups and stabilizing analogous intermediates or transition 19 states. While experimen...