Microbial transglutaminase (mTGase) crosslinking is a useful method for significantly improving the physical functionality of proteins. This study focused on the effect of mTGase modification on the functional properties of yak and cow caseins, including solubility, emulsifying property, water and oil absorption capacity, foaming property, and stability in the presence of Na, Ca, and Mg. The re...

The major milk proteins, the caseins, contain multiple phosphorylation sites. Phosphorylation of the caseins is necessary to allow Ca2+ binding and aggregation of the caseins to form micelles. We have followed the phosphorylation of the caseins in isolated acini from lactating mouse mammary gland. Incubation of mammary cells with [32P]orthophosphate revealed that phosphorylation of newly synthe...

This study aimed to characterize the role of phosphorylation of caseins in selective allergy to goat milk (GM) and sheep milk (SM) in patients with good tolerance to cow milk (CM). We performed skin prick tests with milk and caseins from CM, GM, and SM and immunoblotting and specific immunoglobulin (Ig) E determinations with milk and casein from cow and GM and SM. Sensitization to milk and case...

The separation of whey proteins from caseins has traditionally been carried out by precipitation of the caseins, either through destabilisation by rennet or by acidification. Microfiltration (MF) is a membrane filtration process that has been investigated for the fractionation of milk proteins [1,2,3]. Microfiltration membranes with molecualr weight cutoff (MWCO) 0.1 μm can be used to separate ...

LAUER, B. H., and B. E. BAKER. 1977. Amino acid composition of casein isolated from the milks of different species. Can. J. Zool. 55: 231-236. Casein was isolatedfrom the milks of the following species: cow, horse, pig, reindeer, caribou, moose, harp seal, musk-ox, polar bear, dall sheep, and fin whale. The caseins were subjected to acid hydrolysis, the resultant amino acids were converted to t...

A recent review of clinical studies reports that dairy products may improve inflammation, a key etiologic cardiovascular disease risk factor. Yet the impact of dairy proteins on inflammatory markers is controversial and could be mediated by a differential impact of whey proteins and caseins. In this study, we hypothesized that whey proteins may have a greater anti-inflammatory effect than casei...

Caseins are recognized as safe for consumption, abundant, renewable and have high nutritional value. Casein molecules found in different aggregation states their multiple binding sites offer the potential delivering biomolecules with and/or health benefits, such vitamins, phytochemicals, fibers, lipids, minerals, proteins, peptides, pharmaceutical compounds. In present review, we highlight inte...

It has been shown previously that cultures of mouse mammary epithelial cells retain their characteristic morphology and their ability to produce gamma-casein, a member of the casein gene family, only if they are maintained on floating collagen gels (Emerman, J.T., and D.R. Pitelka, 1977, In Vitro, 13:316-328). In this paper we show: (a) Cells on floating collagen gels secrete not only gamma-cas...

The functionality of casein that was precipitated by batch by a novel process using CO2 was investigated for solubility in water and NaCl, foam stability, overrun, emulsion activity index, and emulsion stability. These properties were compared with those of acidified casein and Na caseinate prepared in the laboratory, commercial acid casein, and commercial Na caseinate and Ca caseinates. The pH...

VÍTKOVÁ M., RAUCH P., FUKAL L. (2002): Optimisation of indirect competitive ELISAs of α-, β-, and κ-caseins for the recognition of thermal and proteolytic treatment of milk and milk products. Czech J. Food Sci., 20: 53–62. Polyclonal antibodies were raised against six immunogens (three native and three thermally treated casein fractions: α+β-casein, κ-casein and whole casein). Using these antib...