Despite major advances in mass spectrometry, the detection of phosphopeptides by liquid chromatography with electrospray mass spectrometry (LC/ES-MS) still remains very challenging in proteomics analysis. Phosphopeptides do not protonate efficiently due to the presence of one or more acidic phosphate groups, making their detection difficult. However, other mechanisms also contribute to the diff...

objective: amorphous calcium phosphate (acp) is a reactive solution capable of releasing calcium and phosphate ions, conversion to apatite and remineralization of mineral tooth structure. this study reviews acp structure, its properties and its applications in dentistry.   methods: in this review study, keywords including “amorphous calcium phosphate”, “mineralization”, “hydroxyapatite”, “casei...

The maize gene RAB-17, which is responsive to abscisic acid, encodes a basic glycine-rich protein containing, in the middle part of its sequence, a cluster of 8 serine residues followed by a putative casein kinase 2-type substrate consensus sequence. This protein was found to be highly phosphorylated in vivo. Here, we show that RAB-17 protein is a real substrate for casein kinase 2. RAB-17 prot...

OBJECTIVES The aim of the systematic literature review is to update the evidence for the prevention of white spot lesions, using materials containing fluoride and/or casein phosphopeptide-amorphous calcium phosphate during and after treatment with fixed orthodontic appliances. MATERIAL AND METHODS Information search for controlled studies on humans published between January 2008 and February ...

Due to the dynamic nature and low stoichiometry of protein phosphorylation, enrichment of phosphorylated peptides from proteolytic mixtures is often necessary prior to their characterization by mass spectrometry. Several phosphopeptide isolation strategies have been presented in the literature, including immobilized metal ion affinity chromatography. However, that technique suffers from poor se...

background and aims. the aim was to evaluate the effect of casein phosphopeptide–amorphous calcium phosphate (cpp-acp) on microtensile bond strength (µtbs) to carious affected dentin (cad) using etch-and-rinse and self-etch adhesive systems. materials and methods. the occlusal surface of 32 human molars with moderate occlusal caries was removed. infected dentin was removed until reaching cad an...

background:dental caries is associated with oral pathogenes and streptococcus mutans (s. mutans)is one of the primary cariogenic organisms. the aim of this clinical study was to evaluate the effect of sugar free chewing gum containing casein phosphopeptide-amorphous calcium phosphate(cpp-acp) and xylitol on salivary streptococcus mutan.   m aterials and methods: 60 dental students, who voluntee...

Casein kinase, which catalyzes the transfer of the terminal phosphate of ATP to dephosphorylated caseins, was prepared from homogenates of lactating bovine mammary tissue by differential centrifugation, followed by extraction of the 66,000 x g particulate fraction with Triton X-100. The enzyme required divalent cations and showed comparable activities with 15 mu Ca’+, 15 mu Mg2+, and 1 mu Mn2’....

  Objective: Amorphous calcium phosphate (ACP) is a reactive solution capable of releasing calcium and phosphate ions, conversion to apatite and remineralization of mineral tooth structure. This study reviews ACP structure, its properties and its applications in dentistry.   Methods: In this review study, keywords including “amorphous calcium phosphate”, “mineralization”, “hydroxyapatite”, “cas...

Selecting an appropriate matrix solution is one of the most effective means of increasing the ionization efficiency of phosphopeptides in matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). In this study, we systematically assessed matrix combinations of 2, 6-dihydroxyacetophenone (DHAP) and diammonium hydrogen citrate (DAHC), and demonstrated that the l...