abstract cyanide is a colorless, harmful, and dangerous substance with a smell similar to bitter almond. cyanide forms a stable compound with iron and magnesium and interferes with cell activity by interfering with respiration, carbon stabilization and nitrate revival. in order to evaluate cyanide metabolism in fuji and abassi mashhad apples, a factorial experiment was conducted as split plot b...

Plants produce cyanide (CN-) during ethylene biosynthesis in the mitochondria and require β-cyanoalanine synthase (CAS) for CN- detoxification. Recent studies show that CAS is a member of the β-substituted alanine synthase (BSAS) family, which also includes the Cys biosynthesis enzyme O-acetylserine sulfhydrylase (OASS), but how the BSAS evolved distinct metabolic functions is not understood. H...

Plants produce cyanide (CN2) during ethylene biosynthesis in the mitochondria and require b-cyanoalanine synthase (CAS) for CN2 detoxification. Recent studies show that CAS is a member of the b-substituted alanine synthase (BSAS) family, which also includes the Cys biosynthesis enzyme O-acetylserine sulfhydrylase (OASS), but how the BSAS evolved distinct metabolic functions is not understood. H...

Protocells containing enzyme-driven biomolecular circuits that can process and exchange information offer a promising approach for mimicking cellular features developing molecular platforms. Here, we employ synthetic transcriptional together with CRISPR/Cas-based DNA processing inside semipermeable protein-polymer microcompartments. We first establish protocell be activated by external strands ...

Aldehyde dehydrogenase 1 (ALDH1) is reported as a biomarker for identifying some cancer stem cells, and down-regulation or inhibition of the enzyme can be effective in anti-drug resistance and a potent therapeutic for some tumours. In this paper, the inhibitory activity, mechanism mode, molecular docking and 3D-QSAR (three-dimensional quantitative structure activity relationship) of curcumin an...

The CRISPR system consists of a guide RNA (gRNA) complementary to target editable DNA sequence and CRISPR-associated endonuclease (Cas). gRNA Cas together form ribonucleoprotein (RNP) complex. guides the enzyme precise site for cutting DNA. In bacteria, leads viral destruction. This ingenious bacterial immune principle has been used design an organism’s genome at locations gene editing purposes...

In order to discover novel probes that may help in the investigation and control of infectious diseases through a new mechanism of action, we have evaluated a library of phenol-based natural products (NPs) for enzyme inhibition against four recently characterized pathogen β-family carbonic anhydrases (CAs). These include CAs from Mycobacterium tuberculosis, Candida albicans, and Cryptococcus ne...

This review describes advancements toward the development of a capillary-assembled microchip (CAs-CHIP) for simultaneous multiple analyte sensing and microchip capillary electrophoresis. Development of such an advanced system relies on several factors such as improving the fluid handling technique, creating new biosensing mechanisms, and integrating different functional capillaries into a singl...

The genome of the protozoan parasite Plasmodium falciparum, the causative agent of the most lethal type of human malaria, contains a single gene annotated as encoding a carbonic anhydrase (CAs, EC 4.2.1.1) thought to belong to the α-class, PfCA. Here we demonstrate the kinetic properties of PfCA for the CO2 hydration reaction, as well as an inhibition study of this enzyme with inorganic and com...

Biocatalytic CO2 sequestration to reduce greenhouse-gas emissions from industrial processes is an active area of research. Carbonic anhydrases (CAs) are attractive enzymes for this process. However, the most active CAs display limited thermal and pH stability, making them less than ideal. As a result, there is an ongoing effort to engineer and/or find a thermostable CA to fulfill these needs. H...