the main objective of this study was assessment of hormonal treatments effects on sperm quality improvement in barbus sharpeyi. results showed differences in effects of treatments with lhrha2, lhrha2 combined with a dopamine antagonist (met), and cpe (carp pituitary extract) on parameters of sperm volume, motility, spermatocrit, biochemical indices of seminal plasma and sperm efficiency on arti...

the effectiveness of pituitary extract from crucian carp (carassius auratus linnaes 1758) in inducing ovulation in veil tail gold fish (carassius auratus gibelio bloch 1783), was examined and the effect of maternal hormone treatment on egg quality in two types of fertilization (fertilization in aquarium and artificial fertilization), were evaluated in 22·c temperature. fish were injected intrap...

Expressed sequence tag (EST) analysis was adopted to address physiological changes after injection of carp pituitary extract for induction of ovulation. ESTs were analyzed from cDNA libraries constructed from mRNA isolated from channel catfish (Ictalurus punctatus) pituitaries before and after induction of ovulation by injection of carp pituitary extract. One hundred randomly picked clones were...

Activin is involved in pituitary hormone regulation and its pituitary actions can be nullified by local production of its binding protein follistatin. In our recent study with grass carp, local release of growth hormone (GH) was shown to induce activin expression at pituitary level, which in turn could exert an intrapituitary feedback to inhibit GH synthesis and secretion. To further examine th...

Somatolactin (SL), a member of the growth hormone/prolactin family, is a pituitary hormone unique to fish models. Although SL is known to have diverse functions in fish, the mechanisms regulating its secretion and synthesis have not been fully characterized. Using grass carp pituitary cells as a model, here we examined the role of insulin-like growth factor (IGF) in SL regulation at the pituita...

Pituitary hormones can act locally via autocrine/paracrine mechanisms to modulate pituitary functions, which represents an interesting aspect of pituitary regulation other than the traditional hypothalamic input and feedback signals from the periphery. Somatolactin, a member of the growth hormone (GH)/prolactin (PL) family, is a pleiotropic hormone with diverse functions, but its pituitary acti...

Wang X, Chu MM, Wong AO. Signaling mechanisms for 2-adrenergic inhibition of PACAP-induced growth hormone secretion and gene expression grass carp pituitary cells. Am J Physiol Endocrinol Metab 292: E1750 –E1762, 2007. First published February 20, 2007; doi:10.1152/ajpendo.00001.2007.—Pituitary adenylate cyclase-activating polypeptide (PACAP) is a potent growth hormone (GH)-releasing factor in ...

Jiang Q, Ko WK, Lerner EA, Chan KM, Wong AO. Grass carp somatolactin: I. Evidence for PACAP induction of somatolactinand gene expression via activation of pituitary PAC-I receptors. Am J Physiol Endocrinol Metab 295: E463–E476, 2008. First published June 3, 2008; doi:10.1152/ajpendo.90385.2008.—Somatolactin (SL), the latest member of the growth hormone/prolactin family, is a novel pituitary hor...

Spermiation was stimulated in the mature grass puffer, Takifugu niphobles, with an injection of human chorionic gonadotropin (HCG) or carp pituitary extract (CPE). Spermatocrit and sperm density were reduced, but milt production was increased in both the HCG and CPE treatment groups relative to those in the control group (P <0.05). These results should be useful for increasing the fertilization...