OBJECTIVES Recently, matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) was successfully applied for the detection of carbapenemase activity directly from Gram-negative colonies. Based on this principle, we evaluated the performance of MALDI-TOF MS for rapid detection of carbapenemase activity directly from positive blood culture vials. METHODS A total...

The emergence of carbapenemase-producing bacteria poses a new challenge in the management of antibiotic therapies for patients. This report describes a new method using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for rapid detection of carbapenemase activity in enterobacteria, Pseudomonas aeruginosa, and Acinetobacter baumannii. In a panel of 78 isolates, inclu...

The NitroSpeed-Carba NP test was used to rapidly detect and discriminate between the different types of carbapenemases (classes A, B, D) within 30 minutes among a collection 202 Pseudomonas sp. strains (mostly aeruginosa). A total 99 carbapenemase–(including enzymes exhibiting weak carbapenemase activity such as several Guyana Extended-Spectrum (GES)-ß-lactamases) 103 non-carbapenemase producer...

The modified Carba NP test presented here may be a valuable tool for laboratories interested in investigating a large number of carbapenemase-producing bacteria in a less-costly way. The test was evaluated against 48 carbapenemase-producing and carbapenemase-non-producing gram-negative bacteria. No false-positive results were obtained, but false-negative results were observed with OXA-23- and G...

Background and Aims: Klebsiella pneumoniae is a gram negative opportunistic pathogen of nosocomial infections that causes pneumonia, urinary tract infection (UTI), meningitis, septicemia and diarrhea. The emergence and spread of carbapenemase producing Klebsiella pneumoniae often cause failure in the treatment of infections. The aim of this study was to evaluate the prevalence of carbapenemase ...

background and objective: the production of carbapenemases especially klebsiella pneumoniae carbapenemase (kpc) is the most important mechanism of enzymatic resistance in isolated enterobacteriaceae such as k. pneumoniae. the purpose of this study was detected of the carbapenemase producer k. pneumoniae strains with phenotypic and genotypic methods. method: out of 800 strains, 270 k. pneumoniae...

OBJECTIVES A growing incidence of pathogens producing carbapenemases has been observed in many countries including Bangladesh. The present study was carried out to determine the presence of carbapenemase producers among uropathogens. MATERIALS AND METHODS A total of 138 Gram-negative uropathogens were isolated and identified by conventional methods and were screened for carbapenemase producti...

The spread of carbapenemase-producing bacteria constitutes a worldwide problem of major importance to public health. The detection of these enzymes is often reported by most European countries and infection control measures are implemented in order to limit their dissemination. Despite the strong evidence indicating the Balkans as a reservoir for carbapenemase-encoding genes, especially for NDM...

OBJECTIVES Infections caused by carbapenemase-producing Enterobacteriaceae are increasing worldwide, especially in ICUs, and have been associated with high mortality rates. However, unequivocally demonstrating causality of such infections to death is difficult in critically ill patients because of potential confounding and competing events. Here, we quantified the effects of carbapenemase-produ...

Class D β-lactamases with carbapenemase activity are emerging as carbapenem-resistance determinants in gram-negative bacterial pathogens, mostly Acinetobacter baumannii and Klebsiella pneumoniae. Carbapenemase activity is an unusual feature among class D β-lactamases, and the structural elements responsible for this activity remain unclear. Based on structural and molecular dynamics data, we pr...