OBJECTIVES To assess the diagnostic performance of 11 commercial PR3- and MPO-ANCA ELISA systems (direct, capture and high sensitive [hs] ELISA). METHODS Sera from 90 patients with AAV (GPA, MPA and CSS) and 20 disease controls (SLE; RA) and healthy individuals were tested for the presence of ANCA by IFT and by different ELISAs for the presence of PR3-and MPO-ANCA, respectively. Furthermore, ...

background : toxoplasmosis is a worldwide endemic disease. in congenitally infected infants and aids patients, toxoplasmosis causes high rates of morbidity and mortality. in these cases antibody detection is difficult; so detection of parasite or its components could be useful tool for early detection and following treatment of the infection. methods : sixty-three balb/c mice were injected intr...

Background: Potency evaluation of rabies vaccine is a cheap, fast, high precision and consistent with ethical values is critical, so researchers have modified a variety of methods such as: National Institute of Health (NIH) method, Single Radial Immunodiffusion (SRID) and so on. The purpose of the present study was to replace an in vitro method consistent with medical ethics criteria instead of...

background: toxoplasma gondii is an apicomplexan parasite which infects a broad range of hosts. the classical diagnosis of toxoplasmosis relies on serological methods. detection of parasite or its components could be useful tool for early diag­nosis of the infection. methods: fifty mice were infected by the intraperitoneal route with 5000 tachyzoites of t. gondii rh strain. five of them sacrifi...

cystic hydatid disease (hydatidosis) is the most serious tape-worm infection prevalent in the cattle and sheep raising area of the world. hydatidosis in man (as an accidental host) caused by infection with the ova containing larval stage of echinococcus spp. in the last decade different techniques have been employed for the serological diagnosis of hydatid disease, such as iha, ifa, elisa, ccle...

hepatitis b virus (hbv) infection is the 10th leading cause of death worldwide. the most important diagnostic and screening marker for hbv infection is hepatitis b surface antigen (hbsag), and the most widely used hbsag screening test is enzyme-linked immunosorbent assay (elisa). in this study, an elisa assay has been developed for detection of hbsag using two novel monoclonal antibodies (mab) ...

Background There is increasing evidence for the role of impaired intestinal permeability in obesity and associated metabolic diseases. Zonulin is an established serum marker for intestinal permeability and identical to pre-haptoglobin2. Here, we aimed to investigate the relationship between circulating zonulin and metabolic traits related to obesity. Methods Serum zonulin was measured by usin...

Rapid detection of soluble F1 capsular antigen in serum, bubo fluid or urine of patients proved to be a valuable tool in the presumptive diagnosis of plague. We evaluated a F1 capsular antigen capture ELISA resembling a commercially available test kit. The minimal detectable concentration was 4 ng/ml. The specificity was 100% when investigating 47 sera from healthy Malagasy subjects and 98.4% w...

background: toxoplasma gondii , the obligate intracellular parasite is life threatening in aids patients. diagnosis of toxoplasmosis is based on serological methods especially increasing of igm and igg titers, but finding of parasite or its components (antigenemia) may be beneficial method in order to detection of acute toxoplasmosis in immunocompromised patients. methods: ninety-four serum sam...

Hepatitis B core Antigen (or HBcAg) is the major component of the core particles of Hepatitis B virus (or HBV). Particles have a size of 27 nm and contain a circular double-stranded DNA molecule, a specific DNA-polymerase and HBcAg. HBcAg is composed of a single polypeptide of about 17 kD that is released upon disaggregation of the core particles; the antigen contains at least one immunological...