background : toxoplasmosis is a worldwide endemic disease. in congenitally infected infants and aids patients, toxoplasmosis causes high rates of morbidity and mortality. in these cases antibody detection is difficult; so detection of parasite or its components could be useful tool for early detection and following treatment of the infection. methods : sixty-three balb/c mice were injected intr...

background: toxoplasma gondii is an apicomplexan parasite which infects a broad range of hosts. the classical diagnosis of toxoplasmosis relies on serological methods. detection of parasite or its components could be useful tool for early diag­nosis of the infection. methods: fifty mice were infected by the intraperitoneal route with 5000 tachyzoites of t. gondii rh strain. five of them sacrifi...

background: toxoplasma gondii , the obligate intracellular parasite is life threatening in aids patients. diagnosis of toxoplasmosis is based on serological methods especially increasing of igm and igg titers, but finding of parasite or its components (antigenemia) may be beneficial method in order to detection of acute toxoplasmosis in immunocompromised patients. methods: ninety-four serum sam...

capture ELISA system was developed for diagnosis of visceral leishmaniasis (VL) using a monoclonal antibody raised against an antigen previously detected in the urine of VL patients. Urine samples from confirmed VL cases from Yemen, Nepal, Spain, Sudan and Brazil were tested in the capture ELISA in comparison with urine samples from endemic and non-endemic areas along with urine samples from pa...

Background: The diagnosis of toxocariasis heavily depends on immunological tests because the number of parasites is usually few in infected tissues, unless they migrate into an organ such as eye. In general, patients with ocular toxocariasis have serum anti- T canis antibody titres that are significantly lower than those with visceral toxocariasis. Objective: To diagnose the asymptomatic toxoca...

capture elisa system was developed for diagnosis of visceral leishmaniasis (vl) using a monoclonal antibody raised against an antigen previously detected in the urine of vl patients. urine samples from confirmed vl cases from yemen, nepal, spain, sudan and brazil were tested in the capture elisa in comparison with urine samples from endemic and non-endemic areas along with urine samples from pa...

background: the diagnosis of toxocariasis heavily depends on immunological tests because the number of parasites is usually few in infected tissues, unless they migrate into an organ such as eye. in general, patients with ocular toxocariasis have serum anti- t canis antibody titres that are significantly lower than those with visceral toxocariasis. objective: to diagnose the asymptomatic toxoca...

background and objectives: a single reactive igg anti-dengue virus elisa test in the absence of igm antibodies or ns1 antigen may denote current infection or past exposure to the virus. to determine whether igg index value can be used to identify true current dengue infection we conducted a prospective observational study. materials and methods: suspected dengue patients (n =1745) were tested i...

Background: Crimean-Congo hemorrhagic fever (CCHF) is an acute viral zoonotic disease, with a mortality rate of 30-50%. There is no approved vaccine or any specific antiviral treatment for CCHF; therefore, the rapid diagnosis seems to be crucial for both efficient supportive therapy and control of infection spread. In this study, the potency of recombinant nucleoprotein of virus expressed in pr...