Calpain, a Ca2+-dependent cysteine protease, plays significant role in gene expression, signal transduction, and apoptosis. Mutations human calpain-5 cause autosomal dominant neovascular inflammatory vitreoretinopathy the inhibition of activity may constitute an effective therapeutic strategy for this condition. Although is ubiquitously expressed mammalian tissues was recently found to be prese...

the present study investigated the calpain gene diversity in iranian one-hump camels (120 camels) using pcr-rflp method. three genotypes aa, ag and gg were observed with the frequencies of 0.02, 0.19 and 0.78, respectively. the frequencies of a and g alleles were 0.12 and 0.88, respectively. the chi-squared test didn’t show significant deviations from hardy-weinberg equilibrium (p<0.05). associ...

Myostatin (MSTN) is an inhibitor of skeletal muscle growth, and a mutation in the gene coding region leads to increased muscling. Calpastatin (CAST) is a specific inhibitor of the ubiquitous calcium-dependent proteases, µ-calpain and m-calpain, found in mammalian tissues. In this study, genomic DNA was extracted from Zandi sheep blood samples. Gel monitoring and spectrophotometer methods were u...

Tenderness is the single most important factor influencing consumer acceptance of meat. The calpain proteolytic system is known to be responsible for the post-mortem tenderization of meat. The purpose of this study was to determine and compare the tensile strength and total calpain activities in different muscles of camel, cattle, sheep and goat. In camels, the effect of age and sex of animal w...

The objective of this study was to examine the effect short term transportation and lairage time on meat quality, myofibrillary structure calpastatin (CAST), µ-calpain (CAPN1), calpain 3 (CAPN3) heat shock protein 70 (HSPA1A) expression levels longissimus dorsi Kıvırcık breed sheep (n=40). Meat quality evaluated by muscle glycogen amounts, pH, temperature, cooking loss, water holding capacity, ...

The number of mammalian calpain protease family members has grown to 14 on last count. Overactivation of calpain 1 and calpain 2 (and their small subunit) has long been tied to acute neurological disorders (e.g. stroke and traumatic brain injury) and recently to Alzheimer's disease. Loss-of-function mutations of the calpain 3 gene have now been identified as the cause of limb-girdle muscular dy...

tenderness is the single most important factor influencing consumer acceptance of meat. the calpain proteolytic system is known to be responsible for the post-mortem tenderization of meat. the purpose of this study was to determine and compare the tensile strength and total calpain activities in different muscles of camel, cattle, sheep and goat. in camels, the effect of age and sex of animal w...

Calpains are ubiquitous intracellular, calcium-sensitive, neutral cysteine proteases. Calpains play crucial roles in many physiological processes, including signaling, cytoskeletal remodeling, regulation of gene expression, apoptosis and cell cycle progression. Calpains have been implicated in many pathologies including muscular dystrophies, cancer, diabetes, Alzheimer's disease and multiple sc...

skNAC (skeletal and heart muscle specific variant of nascent polypeptide-associated complex α) is a skeletal and heart muscle-specific protein known to be involved in the regulation of sarcomerogenesis. The respective mechanism, however, is largely unknown. In the present paper, we demonstrate that skNAC regulates calpain activity. Specifically, we show that inhibition of skNAC gene expression ...

apoptosis, a form of programmed cell death that occurs under physiological as well as pathological conditions, is characterized by morphological and biochemical features. while the importance of caspases in apoptosis is established, several noncaspase proteases (ca2+-dependent proteases) such as calpain may play a role in the execution of apoptosis. the calpain family consists of two major isof...