We analyzed DNA damage, mitotic activity and polyploidization in Crepis capillaris callus cells during shortand long-term in vitro culture, and the influence of plant growth regulators on these processes. Changes in the concentration of growth regulators altered the stability of callus. The level of DNA damage was highly dependent on the growth regulator composition of the medium. Cytokinin at ...

Wild pistachio (Pistacia atlantica subsp. kurdica) is one of tree species that cover much of the forest in northwest of Iran and used in industries, medicine and as food. The present study describes callus induction of the P. atlantica subsp. kurdica using seedling stem explants and the influence of different plant growth regulators including kinetin, benzyladenine (BA), thidiazuron (TDZ), 6Ben...

in this study, responses of four canola genotypes to salt stress in germination test and callus culture stages were investigated. the response of genotypes under salinity levels was significantly different. higher salinity resulted in less germination and growth of seedling. after callus culturing on liquid saline media, callus fresh weight and callus induction efficiency were significantly dif...

the present study was carried out to investigate micropropagation possibility and determine the optimal medium composition and plant growth regulators (pgrs) combinations under in vitro conditions. the cotyledon and epicotyl explants obtained from 1 month old in vitro grown seedlings were used in ms medium containing ba (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) and tdz (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) e...

Through observations of smear materials under a dissecting microscope, it was found that small smooth protuberances were produced in regions showing the dry appearance. Trichomes occurred in the regions showing the green color or the white and green color. There were tracheary elements having thickened wall in the inner part of callus, adjacent to which chloroplasts were observed11>. Scanning e...

background & aim: investigation of suspension cell culture and callus production in catharanthus roseus. catharanthus roseus is an herbaceous perennial plant belongs to the family apocynaceae. c. roseus is important as commercial medicinal plant because of its alkaloidal content. whole plant contains about 130 alkaloids those are classified as vinca alkaloids. vincristine and vinblastine; t...

context : cumin ( cuminum cyminum l.) as a member of the apiaceae family is one of the most important medicinal plants in the world. objective : an experiment was conducted for evaluation of callus induction optimization in cumin accessions (shahdad, koohbanan, badrood and afghanistan). materials and methods : factorial experiment based on completely randomized design (crd) with three replicati...

Studies were conducted on the effect of amino acids and growth regulators on the production of artemisinin, an antimalarial compound, in the callus of Artemisia absinthium. Callus was initiated on solid MS medium supplemented with BAP and NAA from leaf explant. For the production of artemisinin, the callus was proliferated on sterile filter paper bridge in MS medium supplemented with different ...

Agrobacteria mediated Coleus blumei tumour tissues were cultured in vitro on MS medium. Sixteen diversified transformed callus cultures were maintained for several years in the absence of plant growth regulators and antibiotics without affecting the growth rate. Rosmarinic acid was detected spectrophotometrically in all tissue lines but in different quantities. The highest rosmarinic acid accum...