An efficient protocol was developed to control excessive phenolic compound secretion during callus culture of cotton. As cotton is naturally rich in phenolic compounds factors influencing the phenolic compound secretion, callus induction and proliferation were optimized for getting high frequency callus culture. Different carbon sources such as fructose, glucose, sucrose and maltose were tested...

We analyzed DNA damage, mitotic activity and polyploidization in Crepis capillaris callus cells during shortand long-term in vitro culture, and the influence of plant growth regulators on these processes. Changes in the concentration of growth regulators altered the stability of callus. The level of DNA damage was highly dependent on the growth regulator composition of the medium. Cytokinin at ...

effects of culture medium containing hormonal compositions on phenotypic and chromosomal variations of sugarbeet cultured tissues were examined. experiments were carried out in different nacl levels. cytological studies were conducted on calli originated from suspension culture. hypocotyl of seven day old seedlings of the two diploid lines were cultured in ms medium containing hormonal composit...

plant tissue culture techniques were used to study the effect of nacl on tropane alkaloids accumulation and proline content of both datura metel and datura stramonium. callus cultures were established for both datura species. however, ms medium supplemented with 1mg/l of both ba and naa was the best for callus production in d. metel, while ms supplemented with 3 mg/l kin and 1 mg/l 2, 4-d was t...

Perennial ryegrass (Lolium perenne L.) is a widely used cool-season grass species as turf and forage. In order to use biotechnological approaches to improve the species, factors affecting tissue culture responses of mature caryopses of perennial ryegrass were studied. Callus induction and regeneration from mature caryopses of thirteen recentlyreleased turf-type perennial ryegrass cultivars were...

To establish a high-efficiency system of isolated microspore culture for different barley genotypes, we investigated the effects of nitrogen sources and concentrations on callus induction and plant regeneration in different barley genotypes. The results showed that the organic nitrogen sources greatly increased the callus induction, and the great reduction of total nitrogen sources would signif...

An efficient procedure for shoot regeneration was obtained by endosperm culture in Actinidia deliciosa cv. Hayward. Mature endosperm cultured on MS medium supplemented with 2 mg/l 2,4-D and 5 mg/l kinetin developed callus with 80% efficiency. Callus was transferred on MS medium containing different plant growth regulators (2,4-D, TDZ, IAA, NAA, BAP, kinetin, 2iP) for regeneration. There were si...

Crataegus aronia is a wild medicinal plant belonging to the Rosaceae family and has been known for the medicinal activity of its extracts due to the high levels of flavonoids and phenolic compounds. Recently, in vitro culture of plant cells, callus and microshoots is widely used to maximize production of plant secondary metabolites. In this study, the effect of different light intensity levels ...

abstract this experiment was conducted for optimizing a suitable medium for callus induction, regeneration and rooting of anthurium. leaf and petiol explants of antadra and casino varieties were used for callus production. ms medium was supplied with growth regulators (naa, ba, kin and iba). a complete randomized design with 3 replication was used in this expriment. result indicates that variet...