DNA polymerase III from Saccharomyces cerevisiae is analogous to the mammalian DNA polymerase delta by several criteria, including an increased synthetic activity on poly(dA).oligo(dT) (40:1 nucleotide ratio) in the presence of calf thymus proliferating-cell nuclear antigen (PCNA), or cyclin. This stimulation assay has been used to purify the yeast analog of PCNA/cyclin (yPCNA) to homogeneity. ...

Tranexamic acid derivatives modified by amino acids, i.e. tranexamic acid-His, tranexamic acid-Leu-His, tranexamic acid-Glu-Phe, tranexamic acid-Glu-Lys, tranexamic acid-Ser-Tyrand, tranexamic acid-Ser-Phe, were synthesized by Fmoc solid-phase peptide synthesis, purified by reversed-phase high performance liquid chromatography and characterized by 1H and electrospray ionisation mass spectrometr...

The newly synthesized ligand, dihydro OO'bis(salicylidene) 2,2' aminobenzothiazolyl borate (2), was derived from the reaction of Schiff base of 2-aminobenzothiazole and salicylaldehyde with KBH(4). Cu(II) (3) and Zn(II) (4) complexes of (2) were synthesized and further metallated with dimethyltindichloride to yield heterobimetallic complexes (5) and (6). All complexes have been thoroughly chara...

Five coordinated novel complexes of CuII and NiII have been synthesized from benzil and 1,3- diaminopropane-CuII/NiII complex and characterized by elemental analysis, i.r., n.m.r., e.p.r, molar conductance and u.v-vis, spectroscopy. The complexes are ionic in nature and exhibit pentaeoordinated geometry around the metal ion. The reaction kinetics of C25H36N5O2CuCl with calf thymus DNA was studi...

The ability of protein kinase from bovinepineal gland to phosphorylate calf-thymus chromatin and thereby to alter the association between chromatin DNA and histones was investigated. Phosphorylation of calf-thymus chromatin by pineal protein kinase results in an apparent decreased binding between the histones and DNA in chromatin, as indicated by (i) an increase in actinomycin D-binding sites a...

It has been proved that carcinogens bind to macromolecules of the target tissues [ 1,2] and that a positive correlation exists between the extent of binding of hydrocarbons to DNA and their carcinogenic properties [3] . The binding of hydrocarbons is coupled with their metabolic activation [4,5]. The ultimate metabolite of benzo(a)pyrene (BP) as suggested by Boyland [6] has been isolated as a K...

A method for the preparation of biologically active [3H]- and [13C]bleomycin A2 is described. Demethyl Cu(II):bleomycin A2, isolated after pyrolysis of Cu(II):bleomycin A2, was methylated with either [3H]-or [13C]methyl iodide, which resulted in Cu(II):bleomycin A2 labeled in the dimethylsulfonium moiety. Copper was removed by treatment with dithizone in chloroform, and structures were verified...

Simian virus 40 T antigen binds to both single and double strand DNA. The single and double strand DNA binding activity of crude T antigen preparations was evaluated by chromatography of the antigen on DNA-cellulose columns. Crude T antigen was retained on both native and denatured DNA-cellulos columns and was eluted from both columns under similar conditions. The interaction of highly purified...

Hexamethylmelamine (HMM) is metabolized by rat hepatic microsomal preparat ions to reactive species which covalent ly bind to microsomal protein and to calf thymus DNA added to microsomal incubation mixtures. Covalent binding to macromolecules is dependent on the presence of molecular oxygen and reduced nicot inamide adenine dinucleot ide phosphate and is catalyzed by cytochrome P-450 monooxyge...