BACKGROUND Mycological diagnosis of onychomycosis can be performed by direct microcopy (KOH), cultures and calcofluor white. AIMS To compare the percentage of positivity and the degree of correlation of KOH, cultures and calcofluor white for the diagnosis of onychomycosis. METHODS Descriptive, transversal and comparative study. Samples of toenails with onychomycosis were used for KOH, cultu...

BACKGROUND This study aimed to compare three staining methods including: Calcofluor white, Chromotrope and Quick Hot Gram chromotrope used in diagnosis of intestinal microsporidial spores. METHODS One hundred and seventy five stool specimens were collected from patients referred to Laboratory of Intestinal Protozoology at the School of Public Health, Tehran University of Medical Sciences duri...

The flow-injection analysis (FIA) method has long been used for the quantitative determination of b-glucans. FIA makes use of the enhanced fluorescence produced when b-glucan forms complexes with Calcofluor. For successful application of this analysis method, the lowest molecular weight (MW) in the sample has to be higher than a critical MW below which the fluorescence intensity of the complex ...

The spatial pattern of cellular differentiation was studied during fruiting body formation in Polysphondylium pallidum using 3 different staining methods: Calcofluor fluorescence (cellulose accumulation), neutral red (prestalk cells) and immunofluorescence (prespore cells). Neutral-red staining revealed the existence of a clear prestalk region which becomes evident during aggregation and contin...

The growths of Saccharomyces cerevisiae wild-type strain and another strain containing a disrupted structural gene for chitin synthase (chs1::URA3), defective in chitin synthase 1 (Chs1) but showing a new chitin synthase activity (Chs2), were affected by Calcofluor. To be effective, the interaction of Calcofluor with growing cells had to occur at around pH 6. Treatment of growing cells from the...

the present study was carried out for evaluation of calcoflour white staining (cws) as a rapid method for detection of pneumocystis carinii in respiratory specimens of rats as an animal model for human infection. a total of 35 spraque – dawley rats were divided into two groups. group1 (20 rats) received increasing doses of dexamethasone subcutaneously, and group 2(15 rats)as control group that ...

background : this study aimed to compare three staining methods including: calcofluor white, chromotrope and quick hot gram chromotrope used in diagnosis of intestinal microsporidial spores . methods : one hundred and seventy five stool specimens were collected from patients referred to laboratory of intestinal protozoology at the school of public health, tehran university of medical sciences d...

Three monoclonal antibody staining kits, from Genetic Systems, Disease Detection International, and Meridian Diagnostics, were compared with calcofluor white for the direct detection of Pneumocystis carinii in respiratory specimens. Of the 150 specimens tested, 23 were found positive for P. carinii by any of the four stains; 13 were bronchoalveolar lavage, 7 were induced sputum and 3 were expec...

Cover figure: Calcofluor staining illustrating the random budding pattern of ∆twf1/ ∆twf1yeast cells.