confocal laser scanning microscopy (clsm) is a revolutionary advance in the field of light microscopy which, in recent years, has been used on interaction studies between host and pathogen. this study carried out on the flag leaves of the susceptible spring wheat cultivar (triticum aestivum l.) known as thatcher and its near isogenic line with the yr-18 adult-plant resistance gene to stripe rus...

An endothelial cell monolayer with a single mechanically lysed cell was used as a model to examine the extent, kinetics, and nature of local calcium mobilization in the neighborhood of a wound. Individual endothelial cells from confluent monolayers were mechanically lysed with a minutien needle coupled to a micromanipulator while producing no observable mechanical trauma to the neighboring cell...

The whole plant of Biophytum petersianum was extracted with a mixture of water – alcohol (1:1) to evaluate its relaxant effect on aorta rings. In isolated Wistar rat tissue, the hydro-ethanolic extract (0.1; 0.25 and 0.5 mg/ml) non-competitively antagonized calcium chloride and high-K + - induced aorta contractions in a concentration-dependent manner. Moreover, the inhibition of noradr...

This study examined whether propofol and aminophylline affect the mobilization of intracellular calcium in human umbilical vein endothelial cells. Intracellular calcium was measured using laser scanning confocal microscopy. Cultured and serum-starved cells on round coverslips were incubated with propofol or aminophylline for 30 min, and then stimulated with lysophosphatidic acid, propofol and a...

the whole plant of biophytum petersianum was extracted with a mixture of water – alcohol (1:1) to evaluate its relaxant effect on aorta rings. in isolated wistar rat tissue, the hydro-ethanolic extract (0.1; 0.25 and 0.5 mg/ml) non-competitively antagonized calcium chloride and high-k + - induced aorta contractions in a concentration-dependent manner. moreover, the inhibition of noradrenaline–i...

Corticotropin-releasing hormone (CRH) affects cytosolic calcium ion levels. The aim of the present work was to examine the role of protein kinase A (PKA)- and PKC-dependent signalling pathways in mediating the effect of CRH on calcium ion influx (from extra-cellular sources) and calcium ion mobilization (from intra-cellular stores). In this study, we employed a well-known model of neural crest-...

During development, the chick embryo mobilizes the calcium it needs from two extraembryonic sources, first the yolk and then the eggshell. Since previous studies have strongly suggested that vitamins D and K may regulate chick embryonic calcium metabolism, we have examined here how these vitamins might be involved in regulating the calcium mobilization processes. We used as our experimental sys...

During development, the chick embryo mobilizes the calcium it needs from two extraembryonic sources, first the yolk and then the eggshell. Since previous studies have strongly suggested that vitamins D and K may regulate chick embryonic calcium metabolism, we have examined here how these vitamins might be involved in regulating the calcium mobilization processes. We used as our experimental sys...

Vasopressin (AVP), oxytocin (OT) and related peptides induce differentiation and hypertrophy in myogenic cells expressing the V1a-vasopressin receptor (V1aR) or the oxytocin receptor (OTR). Either receptor can transduce both ligand signals. Binding of AVP and OT to the V1aR the target cells activates phosphatidylinositol hydrolysis, which in turn releases Ca 2+ from internal stores. The AVP-dep...

It has been shown in our previous study that melanoma cells induce junction disassembly in the manner related to phospholipase C-calcium activation. In light of this observation, we have developed a mathematical model of the signaling pathway and adapted multi-parametric sensitivity analysis (MPSA) to identify important parameters in the model, which examines tumor cell-induced calcium mobiliza...