background and objectives: in the original in vitro method for evaluation of iron bioavailability using caco-2 cell model, ferritin formation by cells is considered as a functional indicator of both absorption and utilization of iron. however, as iron is measured in the tested material and then ferritin concentration is measured in caco-2 cell lysate, it is almost impossible to have a concrete ...

A series of arylketo-containing P1-P3 linked macrocyclic BACE-1 inhibitors were designed, synthesized, and compared with compounds with a previously known and extensively studied corresponding P2 isophthalamide moiety with the aim to improve on permeability whilst retaining the enzyme- and cell-based activities. Several inhibitors displayed substantial increases in Caco-2 cell-based permeabilit...

conclusions this research study proved the presence of viable probiotic lab microflora in the ewe milk with enhanced health benefits. the 2 selected lactobacillus strains could be exploited in dairy or pharmaceutical industry in future. objectives the probiotic efficacy of two lactobacillus strains namely lactobacillus pentosus lp05 and l. brevis lb32 was evaluated. they were previously isolate...

the uptake and transport of 9-nitrocamptothecin (9-nc), a potent anticancer agent, across caco-2 cell monolayers was studied as a free and plga nanoparticle loaded drug. different sizes (110 to 950 nm) of 9-nitrocamptothecin nanoparticles using poly (lactic-glycolic acid) were prepared by via the nanoprecipitation method. the transport of nanoparticles across the caco-2 cell monolayer as a func...

To increase the intestinal delivery of dietary β-carotene, there is a need to develop nanostructured food systems encapsulate this fat soluble bioactive. The aim study was evaluate bioacessibility and bioavailability across barrier β-carotene-enriched nanoemulsions stabilised with two emulsifiers (lecithin or sodium caseinate) by coupling an in vitro gastrointestinal digestion cell culture mode...

The Caco-2 cell line is well established as an in vitro model for iron absorption. However, the model does not reflect the regulation of iron absorption by hepcidin produced in the liver. We aimed to develop the Caco-2 model by introducing human liver cells (HepG2) to Caco-2 cells. The Caco-2 and HepG2 epithelia were separated by a liquid compartment, which allowed for epithelial interaction. F...

The absorption characteristics of temocapril were investigated using Caco-2 cells, and the esterases expressed in Caco-2 cells were identified. Temocapril was almost completely hydrolyzed to temocaprilat during transport across Caco-2 cells. Hydrolysis experiments of temocapril in Caco-2 cell 9000g supernatant (S9) and brush-border membrane vesicles showed that temocapril was mainly hydrolyzed ...

PURPOSE To predict Caco-2 permeability is a valuable target for pharmaceutical research. Most of the Caco-2 prediction models are based on commercial or special software which limited their practical value. This study represents the relationship between Caco-2 permeability and molecular descriptors totally based on open source software. METHODS The Caco-2 prediction model was constructed base...

Coenzyme Q10 (CoQ10) is a key component of the mitochondrial electron transfer chain and is one of the most important antioxidants. We previously found that glycoprotein prosaposin (Psap) binds CoQ10 in human cells. Although Psap is expressed in the intestines, its role in the gastrointestinal tract is not clear. To elucidate the role of Psap in the intestines, we established Psap knockdown (KD...

Ma, Thomas Y., Michel A. Boivin, Dongmei Ye, Ali Pedram, and Hamid M. Said. Mechanism of TNFmodulation of Caco-2 intestinal epithelial tight junction barrier: role of myosin light-chain kinase protein expression. Am J Physiol Gastrointest Liver Physiol 288: G422–G430, 2005; doi:10.1152/ajpgi.00412.2004.—TNFplays a central role in the intestinal inflammation of various inflammatory disorders inc...