نتایج جستجو برای: c2xs colii e
تعداد نتایج: 1016920 فیلتر نتایج به سال:
: هدف از این تحقیق کلون کردن ژن سازنده ناحیه ثابت زنجیرµ ایمونوگلوبولین ماکیان و بررسی بیان آن در e. coliبود. در این مطالعه در ابتدا توالی ژن سازنده ناحیه ثابت زنجیرµ ایمونوگلوبولین ماکیان از بانک ژن استخراج و مورد بررسی قرار گرفت تا پرایمرهای مناسب برای تکثیر این ژن جهت rt-pcr طراحی گردد. از بافت طحال یک جوجه تازه کشتار شده rna به عنوان الگوی rt-pcr استخراج گردید.با استفاده از پرایمرهای طراحی ...
Quick and simple methods to detect the type of life threatening microbial species are very essenti al in food and water contaminated with bacteria. Recently, by utili zi ng the semiconducting polymeric thin films prepared on si licon macroporous substrates a new technology for detection of micro-organisms has been developed. The advantages of the polymeric devices are the ease of fabrication , ...
Degenerative cranial cruciate ligament (CCL) rupture is characterized histologically by degenerating extracellular matrix (ECM) and chondroid metaplasia. Here, we describe the progression of chondroid metaplasia and the changes in the expression of ECM components in canine CCL rupture (CCLR). CCLs from 26 stifle joints with CCLR (CCLR group) and normal CCLs from 12 young beagles (control group)...
Extracellular phosphate plays a key role in growth plate maturation by inducing Erk1/2 (Mapk3/1) phosphorylation, leading to hypertrophic chondrocyte apoptosis. The Raf kinases induce Mek1/2 (Map2k1/2) and Erk1/2 phosphorylation; however, a role for Raf kinases in endochondral bone formation has not been identified. Ablation of both A-Raf (Araf) and B-Raf (Braf) in chondrocytes does not alter g...
peptidoglycan layer of the bacterial cell wall,3) and accordingly can be a target fbr development of new antibiotics. We purified the enzyme from Pediococcus pentosaeeus4) and cloned its gene into Eycherichia colii'2} to construct an overproducer,S) We here report that L-serine O-sulfate, an analog of L-glutamate, serves as a suicide substrate to inactivate the enzyme, This is thc first example
Cartilage extracellular matrix (ECM) is composed primarily of the network type II collagen (COLII) and an interlocking mesh of fibrous proteins and proteoglycans (PGs), hyaluronic acid (HA), and chondroitin sulfate (CS). Articular cartilage ECM plays a crucial role in regulating chondrocyte metabolism and functions, such as organized cytoskeleton through integrin-mediated signaling via cell-mat...
The purpose of this study was to obtain Escherichia coli isolates from 11 se’i meat sellers in Kupang City and observe their resistance to13 types antibiotics. were isolated identified based on SNI 2897: 2008. Antibiotic the colii determined using Kirby-Bauer method. results showed that 13 (39.39%) samples had a high level erythromycin (100%), tetracycline (76.92%), doxycycline (61.54%). Isolat...
Abstract Background Animal model of Knee Osteoarthritis (OA) is the primary testing methodology for studies on pathogenic mechanisms and therapies human OA disease. Recent major modeling methods are divided into artificially induced spontaneous. However, these have some disadvantages slow progression, high cost no correlation with pathogenesis OA. Methods Our attempted to find a rapid, easy, co...
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