The subgroup II luteovirus barley yellow dwarf virus-RPV (BYDV-RPV) acts as a helper virus for a satellite RNA (satRPV RNA). The subgroup II luteovirus beet western yellows virus (BWYV) and the ST9-associated RNA (ST9a RNA), a BWYV-associated RNA that encodes a polymerase similar to those of subgroup I luteoviruses, were assayed for their ability to support replication of satRPV RNA. SatRPV RNA...

uring the 1980s, crisphead lettuce, butterhead lettuce, and spinach crops in western Oregon (Fig. 1) became increasingly damaged by beet western yellows luteovirus (BWYV) (5,8) infection (15), a previously insignificant problem in this area. Utilizing double antibody sandwich enzyme-linked immunosorbent assay (DASELISA) methodology (2), we identified potential BWYV inoculum sources in vicinitie...

The molecular determinants of stimulation of -1 programmed ribosomal frameshifting (-1 PRF) by RNA pseudoknots are poorly understood. Sugarcane yellow leaf virus (ScYLV) encodes a 28-nt mRNA pseudoknot that promotes -1 PRF between the P1 (protease) and P2 (polymerase) genes in plant luteoviruses. The solution structure of the ScYLV pseudoknot reveals a well ordered loop 2 (L2) that exhibits con...

Aphid transmission of poleroviruses is highly specific, but the viral determinants governing this specificity are unknown. We used a gene exchange strategy between two poleroviruses with different vectors, Beet western yellows virus (BWYV) and Cucurbit aphid-borne yellows virus (CABYV), to analyze the role of the major and minor capsid proteins in vector specificity. Virus recombinants obtained...

beet western yellows virus (bwyv), a species of the genus polerovirus in the family luteoviridae, is an agriculturally important virus infecting over 150 plant species in 23 dicotyledonous families worldwide. a survey of bwyv in canola fields in golestan and tehran provinces of iran using indirect triple antibody sandwich enzyme-linked immunosorbent assay (tas-elisa) indicated 8.3 % infection. ...

turnip mosaic virus (tumv), cauliflower mosaic virus (camv) and beet western yellow virus (bwyv) are considered as the most important viruses of canola, worldwide. in order to determine the distribution of these viruses in golestan province, samples from three important canola growing areas of the province were collected during 2008 and 2009. the randomly collected samples were tested for the p...

The genomic RNA of beet western yellows virus (BWYV) contains a potential translational frameshift signal in the overlap region of open reading frames ORF2 and ORF3. The signal, composed of a heptanucleotide slippery sequence and a downstream pseudoknot, is similar in appearance to those identified in retroviral RNAs. We have examined whether the proposed BWYV signal functions in frameshifting ...