background: brucellosis is one of the most important zoonosis that is prevalent among human and animal. today, a large percentage of animal and human population suffer from its side effects. objectives: the purpose of the present study was to estimate the prevalence of anti-brucella antibodies in flock- and animal-level in districts of south of kerman province. methods: in this cross-sectional ...

Rose-Bengal test, complement fixation test and the agglutination are mainly used for diagnosis of animal brucellosis. These tests characterized by low sensitivity specificity, which is one main reasons effectiveness measures aimed at eradicating The use modern highly sensitive serological requires availability antigens specific to Brucella spp. aim study was obtain multiproteins pathogen recomb...

Group 3 of outer membrane proteins (OMPs) of Brucella includes Omp25 and Omp31, which share 34% identity. Omp25 is highly conserved in Brucella species, and Omp31 is present in all Brucella species, except Brucella abortus. Antibodies to Brucella melitensis Omp31 have been sought only in infected sheep, and Western blotting of sera from infected sheep did not reveal anti-Omp31 reactivity. We ob...

Arctic foxes (Vulpes lagopus) are susceptible to smooth Brucella (s-Brucella) infection and may be exposed such bacteria through the consumption of infected marine mammals, as implied by finding s-Brucella antibodies in polar bears (Ursus maritimus). Svalbard have not previously been investigated for s-Brucella antibodies, but antibodies detected Iceland, Alaska (USA) Russia...

This cross-sectional study was conducted to estimate seroprevalence and to identify flock-level factors associated with seropositivity to brucellosis in small ruminants in Kerman province, southeastern Iran. In October-November 2011, serum samples were randomly collected from 1767 sheep and 1233 goats, older than 18 months, from 300 flocks. The sera were initially screened for the presence of a...

In this study, a localized surface plasmon resonance (LSPR) nanobiosensor was designed to quantify anti-Brucella antibodies in the human sera. Smooth Lipopolysaccharide (LPS) extracted from Brucella melitensis via modified hot phenol water method and fixed on of gold nanoparticles by covalent interactions with functionalized nanoparticles. To obtain best performance probe, ratio LPS nanoparticl...

OBJECTIVE 1) To identify patients of brucella spondylitis and sacroiliitis amongst patients of 'infective spondylitis' and 'sacroiliitis', 2) To study clinical and radiographic features and outcome of brucella spondylitis. METHODS We reviewed 21 patients of infective spondylitis and 12 of sacroiliitis attending our hospital between March and September 2000 and followed them to identify patien...

Brucellosis is a zoonotic disease which can be transmitted by direct or indirect contact with infected animal or their products. It is an important public health problem but little is known on brucellosis in the Malaysian population. The aim of this study was to determine the presence of Brucella antibodies using commercial Brucella IgG and IgM ELISA kits (Vircell, SL, Barcelona Spain). A total...

this cross-sectional study was conducted to estimate seroprevalence and to identify flock-level factors associated with seropositivity to brucellosis in small ruminants in kerman province, southeastern iran. in october-november 2011, serum samples were randomly collected from 1767 sheep and 1233 goats, older than 18 months, from 300 flocks. the sera were initially screened for the presence of a...

OBJECTIVES This study was conducted in Northern Italy with the aim of defining the risk of agricultural workers' contact with biological agents through the determination of serum antibodies against selected zoonotic agents. Immunity against tetanus was also investigated. METHODS Two groups of agricultural workers consisting of 153 animal breeders (exposed) and 46 non- breeders (controls) were...