AIMS We assessed the veracity of intergenic spacer region 1 (ITS1) ribotyping for the rapid, inexpensive and accurate identification of Brenneria goodwinii and Gibbsiella quercinecans that are associated with acute oak decline (AOD) in the UK. METHODS AND RESULTS Agarose gel electrophoresis and polyacrylamide gel electrophoresis (PAGE) were applied for the typing of ITS1 PCR amplicons from st...

Brenneria goodwinii is one of the most frequently isolated Gram-negative bacteria from native oak species, Quercus robur and Q. petraea, affected by acute oak decline (AOD) in the UK. We investigated the population biology of this bacterial species using a multilocus sequence analysis to determine the population structure and evolutionary potential. Seven partial housekeeping genes were used in...

The decline of oak stands is a phenomenon that has been observed since the beginning 20th century in many European countries. It can be caused both with abiotic factors such as drought and fluctuations groundwater levels, well biotic infestations by insects, fungi bacteria. Acute Oak Decline (AOD) an dangerous disease trees which was first XX century. From moment symptoms were noticed, able to ...

L3-3HAT, a Gram-negative-staining, facultatively anaerobic, motile bacterial strain, was isolated from the symptomatic bark of Salix matsudana canker in China. 16S rRNA gene analysis revealed that novel strain shares highest sequence similarity with Brenneria goodwinii FRB141T (95.5%). In phylogenetic trees based on four housekeeping genes (gyrB, rpoB, atpD, and infB) sequence, formed separate ...

Abstract Acute Oak Decline (AOD) is a complex plant disease affecting species of native oaks, for example, Quercus robur , in Latvia. Typical AOD symptoms are bark cracks with dark exudate. In other European region countries, like the United Kingdom, associated insects, Agrilus biguttatus . From results forest survey, it can be concluded that oak stands located Latvia, there practically no dama...

Brenneria species are bacterial plant pathogens mainly affecting woody plants. Association of all members with devastating disorders (e.g., acute oak decline in Iran and United Kingdom) due to adaptation pathogenic behavior response host environmental factors. Some species, including B. goodwinii, salicis, nigrifluens, also show endophytic residence. Here we that novel sp. closely related. Gene...

Acute oak decline (AOD) is a relatively newly described disorder affecting native oak species in Britain. Symptomatic trees are characterised by stem bleeds from vertical fissures, necrotic lesions in the live tissue beneath and larval galleries of the two spotted oak buprestid (Agrilus biguttatus). Several abiotic and biotic factors can be responsible for tree death, however the tissue necrosi...

Outbreaks of acute oak decline (AOD) have been documented in England from 2006. Both species of native oaks (Quercus robur and Quercus petraea) are affected. To complement isolation efforts for identification of putative causative biotic agents and increase our understanding of bacteria associated with oak tissue, five sites in England were chosen for this study. Samples of outer bark, inner ba...