background: tumour suppressor genes such as tp53, brca1 and rad51 are involved in dna repair and their malfunctions result in genomic instability and cancer. wild type (wt) tp53 binds to brca1and rad51 in vivo and in vitro. however, mutated tp53 in tumours can interfere with wt tp53 function. we studied how mutation of tp53 in mda-mb-468 cell line could affect its binding capacity and interfere...

objective: cd44+/cd24-/low breast cancer cells have tumour-initiating properties with stem cell-like features. breast cancer gene 1 (brca1) is a tumour suppressor gene that plays a crucial role in dna repair and maintenance of chromosome stability. the clinicopathological features of breast cancer in brca1 mutation carriers suggest that brca1 may function as a stem-cell regulator. materials and...

background: breast cancer is the most common malignancy in iranian women. mutations in brca1 gene is one of the important genetic predisposing factors in breast cancer. this gene is a tumor suppressor that plays an important role in regulating the functions of rad51 protein for strand invasion in homologous recombination repair. methods: the brca1 gene has amplified in the dna isolated from bre...

Introduction: Mutations in the BRCA1 gene are major risk factors for breast and ovarian cancers. However, the relationship between some BRCA1 mutations and cancer risk remains largely unknown. Cancer risk predictions could be improved by evaluation of the impairment degree in the BRCA1 functions due to a specific mutation. This study aimed to assess the functional effect of a novel variant (Glu...

Introduction: Mutations in the BRCA1 gene are major risk factors for breast and ovarian cancers. However, the relationship between some BRCA1 mutations and cancer risk remains largely unknown. Cancer risk predictions could be improved by evaluation of the impairment degree in the BRCA1 functions due to a specific mutation. This study aimed to assess the functional effect of a novel variant (Glu...

BRCA1 is a breast cancer susceptibility gene that is down-regulated in a significant proportion of sporadic breast cancers. BRCA1 is posttranscriptionally regulated by RNA-binding proteins, the identities of which are unknown. HuR is an RNA binding protein implicated in posttranscriptional regulation of many genes and is overexpressed in sporadic breast cancer. To investigate the possibility th...

BRCA1 is a tumor suppressor gene that is responsible for hereditary breast and ovarian cancer syndrome. To clarify the possible involvement of the BRCA1 protein in mammary carcinogenesis in sporadic and hereditary forms, we have analyzed the BRCA1 protein expression pattern in five breast epithelial cell lines, including a BRCA1-deficient cell line, and 162 breast cancer tissue samples [includi...

BRCA1 is a tumor suppressor gene that is responsible for hereditary breast and ovarian cancer syndrome. To clarify the possible involvement of the BRCA1 protein in mammary carcinogenesis in sporadic and hereditary forms, we have analyzed the BRCA1 protein expression pattern in five breast epithelial cell lines, including a BRCA1-deficient cell line, and 162 breast cancer tissue samples [includi...