enrichment of cell suspension with germ cells prior to injection into recipient seminiferous tubules is of importance in spermatogonial stem cells (sscs) transplantation. knock-out serum replacement (ksr) has been reported to enhance the proliferation of murine sscs and human embryonic stem cells. the aim of the present study was to investigate the effect of ksr versus fetal bovine serum (fbs) ...

conclusions the newly developed simple indirect elisa showed high sensitivity and specificity with respect to vnt. developing such a simple, sensitive, and specific elisa which is much less expensive than the available commercial elisa kits can improve the detection of bvdv infections, help to eliminate the disease from herds, and decrease economic losses caused by this disease. background bovi...

background: the aim of the study was to provide a point of reference to study the neospora caninum infections in china. methods: genome dna was extracted from the brains of aborted fetuses and specific pcr was performed with n. caninum nc5-targeted specific primers. fetal bovine brain tissues were homogenized and continuously cultured in vero cells with double antibodies. the medium was replace...

3450 bovine from different breeds and ages were clinically examined for the presence of arthritis. 120 cases showed macroscopic evidences of arthritis. mycoplasma spp were isolated from synovial fluid of 21 affected animals. in addition to mycoplasma spp. arcanobacterium pyogenes and staphylococcus epidermidis were isolated from 2 cases. the synovial fluid was markedly increased, straw color, t...

background: many studies reported that follicle size has an essential role in developmental potential of oocytes. also, the brilliant cresyl blue (bcb) test is one of the most important criteria in selection of more competent oocytes. objective: selection of developmentally competent bovine oocytes. materials and methods: a total of 1730 bovine cumulus oocyte complexes (cocs) were recovered fro...

In this syudy a continuous bovine kidney cell line derived from a primary bovine kidney cells was established for the first time in Iran. The cells were originating from two-day-old normal male calf of Holstein breed. The cell cultures were continuously passaged following complete proliferation of primary cells. The specific properties or characteristics of the cell were defined using cytogenet...

background: the aim of this study was identification of the epidemiology of prototheca zopfii species from the milk samples of dairy cattle in isfahan, central iran. methods: milk samples were obtained from 230 dairy cattle, 130 with and 100 without mastitis, in isfahan. the samples were cultured in prototheca isolation medium (pim) and sabouraud's dextrose agar. all p. zopfii isolates were ide...

background: niche cells, regulating spermatogonial stem cells (sscs) fate are believed to have a reciprocal communication with sscs. the present study was conducted to evaluate the effect of ssc elimination on the gene expression of glial cell line-derived neurotrophic factor (gdnf), fibroblast growth factor 2 (fgf2) and kit ligand (kitlg), which are the main growth factors regulating sscs deve...