introduction: dna extraction methods are very important for genetics research and diagnostic tests. in these methods are also important for detecting genetic diseases or in cancers for investigating genetic changes during cancer progression or treatment. therefore, selection of the best method for dna extraction from different samples such as bone marrow (bm) or peripheral blood (pb) and their ...

The purpose of this study was to investigate the roles of bcl-2 chromosomal translocation and Bcl-2 protein expression in follicular lymphoma (FL) minimal bone marrow (BM) infiltration. We identified the same bcl-2/ IgH fusion gene in paraffin-embedded lymph node (LN) samples and BM samples using immunohistochemistry (IHC), immunocytochemistry (ICC), cytologic morphology and fluorescence in sit...

BACKGROUND 14q32 rearrangement has been identified as a recurrent hotspot of translocations in multiple myeloma (MM). The Fluorescence Immunophenotyping and Interphase Cytogenetics as a tool for the Investigation of Neoplasms (known as FICTION technique) for evaluation of chromosomal changes in MM. The aim of this work is to detect 14q32 rearrangement, using FICTION technique, on archival bone ...

Background. FISH is a molecular cytogenetic technique enabling rapid detection of genetic abnormalities. Facilities that can run fresh/wet samples for molecular diagnosis and monitoring of neoplastic disorders are not readily available in Ghana and other neighbouring countries. This study aims to demonstrate that interphase FISH can successfully be applied to archival methanol-fixed bone marrow...

Background/aim: Ovarian transplantation can preserve fertility in cancer patients. The aim of this study was to compare ovarian transplantation into granulation tissue (GT) versus back muscle (BM) sites and also to investigate the effects of N-acetylcysteine (NAC) as an antioxidant on ovarian survival in a rat autograft model.Materials and methods: Twenty-eight adult female rats were divided in...

BACKGROUND/AIMS Epstein-Barr virus (EBV) is involved in the pathogenesis of angioimmunoblastic T-cell lymphoma (AILT), but its precise role and prognostic impact are not clear. This study aimed to evaluate the incidence of EBV-postitivity in the tumor and bone marrow (BM) samples from AILT patients, and their correlations with the clinical variables and patient survival. METHODS Seventy AILT ...

Abstract INTRODUCTION Little is known about tumour-infiltrating lymphocytes (TILs) in brain metastasis (BM) and their utility as predictive biomarker for immunotherapy. AIM: To analyze TILs BM, correlate with corresponding paired primary (PP). METHODS Inclusion: Histologically confirmed BM PP (2015-2020). Exclusion: Hematological malignancies, spinal cord/meningeal metastasis, pediatric age, sl...

Neuroblastoma is the most common extra-cranial solid tumor in childhood. Presence of disseminated tumor cells (DTCs) in the bone marrow (BM) at diagnosis and at relapse is a common event in stage M neuroblastomas. Although the clinical heterogeneity of disseminated neuroblastomas is frequently associated with genomic diversity, so far, only little information exists about the genomic status of ...

background: magnetic resonance imaging (mri) is a safe modality and useful in characterizing normal and abnormal bone marrow (bm). mri also presents a more global view of bm than biopsy therefore, it may provide a better understanding of hematologic disorders. the purpose of this study was to monitor radiation-induced alterations of bm in acute phase of irradiation (1-10 day after total body ir...