The in situ expression of intercellular adhesion molecule-1 (ICAM-1) mRNA in normal and pneumonic lung tissues of Holstein calves with bovine leukocyte adhesion deficiency (BLAD) was compared with that of age-matched non-BLAD Holstein calves by in situ hybridization. Twenty-four Holstein calves (both BLAD and non-BLAD) were randomly assigned to one of two experimental groups and inoculated intr...

Using transposon-mediated gene-trap mutagenesis, we have generated a novel mouse mutant termed Blad (Bloated Bladder). Homozygous mutant mice die perinatally showing a greatly distended bladder, underdeveloped diaphragm and a reduction in total skeletal muscle mass. Wild type and heterozygote mice appear normal. Using PCR, we identified a transposon insertion site in the first intron of Nmnat2 ...

BACKGROUND Bovine leukocyte adhesion deficiency (BLAD), deficiency of uridine monophosphate synthase (DUMPS), complex vertebral malformation (CVM), bovine citrullinaemia (BC) and factor XI deficiency (FXID) are autosomal recessive hereditary disorders, which have had significant economic impact on dairy cattle breeding worldwide. In this study, 350 Holstein cows reared in Turkey were screened f...

Among the various hereditary diseases that have been widely studied in dairy cattle, bovine leukocyte adhesion deficiency (BLAD), deficiency of uridine-5-monophosphate synthase (DUMPS), and complex vertebral malformation (CVM) are noteworthy because of their high impact on overall herd productivity as a consequence of increased calf mortality. The aim of this study was to verify the frequency o...

Bu çalışmada Türkiye’deki Holştayn melezlerinde Sığır Lökosit Bağlanma Eksikliği (BLAD) ve Faktör XI (FXID) kalıtsal hastalıklarına sebep olan mutant allellerin varlığının araştırılması amaçlanmıştır. Çalışmada toplam 48 baş sığır incelenmiştir. Yapılan moleküler analizlerden sonra incelenen melez sığırların birinin BLAD taşıyıcısı olduğu, FXID bireye rastlanılmadığı görülmüştür. çalışma ile bu...

Bovine Leucocyte Adhesion Deficiency (BLAD) is a Holstein-Friesian HF) specific autosomal recessively inherited disorder characterized by recurrent pneumonia, ulcerative and granulomatous stomatitis, enteritis, periodontitis, loss of teeth, delayed wound healing, persistent neutrophilia and death at an early age [1]. BLAD is a disease characterized by a reduced expression of the adhesion molecu...

Livestock species can be affected by different types of genetic defects. Recently, we are faced with genetic defects in dairy cattle such as bovine leukocyte adhesion deficiency (BLAD) and complex vertebral malformation (CVM) both are autosomal recessive defects leads to an inherited lethal disease in Holstein breed. BLAD is caused by a deficiency in leukocyte surface glycoproteins known as int...

Bovine leukocyte adhesion deficiency (BLAD) is a genetically transmitted disease and inherited as an autosomal recessive defect. Keeping this in view the present study was conducted to estimate incidence of carrier animals in a herd of breeding bulls for mutation in Integrin â2 gene, reared at Frozen Semen Bull Station (FSBS), Haringhata, Nadia, West Bengal, India. A total of 80 bulls of differ...

A screening was carried out for CVM, BLAD, DUMPS, bovine citrullinaemia, glycogen storage disease V, and Robertsonian translocations in the cattle population of the Czech Republic. In 406 Holstein sires and 146 Czech Pied (Czech Simmental) sires entering the AI programme in the Czech Republic from 2003–2005, no heterozygous sire for DUMPS, bovine citrullinaemia and BLAD was found. The heterozyg...

BACKGROUND During seed germination, beta-conglutin undergoes a major cycle of limited proteolysis in which many of its constituent subunits are processed into a 20 kDa polypeptide termed blad. Blad is the main component of a glycooligomer, accumulating exclusively in the cotyledons of Lupinus species, between days 4 and 12 after the onset of germination. PRINCIPAL FINDINGS The sequence of the...