We selected a novel biotin-binding peptide for sensing biotin, biotinylated proteins, and nucleotides. From 15-mer library displayed on the RNA coliphage Qβ, 15-amino acid long (HGHGWQIPVWPWGQG) hereby referred to as nanotag was identified selectively bind biotin. The target selection achieved through panning with elution by infection. tested transducer an immunogenic epitope of foot-and-mouth ...

Chitosan diffraction-based leaky waveguides were shown to have good reproducibility and be suitable for monitoring the binding of anti-biotin antibody biotin in presence physiological levels serum albumin.

how to cite this article: kohmanaee sh, zarkesh m, tabrizi m, hassanzadeh rad a, divshali s, dalili s. biotinidase deficiency in newborns as respiratory distress and tachypnea: a case report. iran j child neurol. spring 2015; 9(2):58-60. abstract objective biotin is a coenzyme composed of four carboxylases. it presents in amino acid catabolism, fatty acid synthesis, and gluconeogenesis. biotini...

The high affinity of biotin to streptavidin has made it one the most widely used tags in proteomics. Early methods for enrichment alone and mostly ignored biotin-labeled peptide. Recent advances labeling have led an increase biotinylation efficiency shifted interest detection site biotinylation. This increased confidence identification provided additional structural information, yet requires ef...

Laser-deposited gold immobilization layers having different porosities were incorporated into love wave surface acoustic sensors (LW-SAWs). Variation of pulsed laser deposition parameters allows good control the film morphology. Biosensors with various tested using biotin–avidin reaction. Control Au layer morphology is important since biotin and avidin morphologies closely follow that gold. The...

multiple sclerosis is an autoimmune disease caused by damage to the myelin of the nerve cells in the spinal cord and brain, ms was classified into 4 types including: relasping/remitting (rr) primary/progressive (pp), secondary/progressive (sp), progressive/relapsing (pr). pr ms is one of the severe forms of ms that lead to inflammation associated physical, mental and vision dysfunction. because...

biotin is a water-soluble vitamin and co-factor for activation of carboxylases apoenzymes. biotinidase enzyme is essential for release of biotin from apoenzymes. absence of biotinidase is an autosomal recessive trait with a prevalence of 1 in 60000. clinical manifestations of biotinidase deficiency include dermatitis, alopecia, seizures, hypotonia, developmental delay, hearing loss, visual impa...

Biotin is a water-soluble B complex vitamin and coenzyme of five types carboxylase plays crucial roles in fatty acid, glucose, amino acid metabolism. Nutritional biotin deficiency defective enzymes essential for metabolism cause inflammatory diseases such as eczema-like dermatitis Crohn’s disease; however, little known about the pathophysiological biotin. This study investigated relationship be...

Background: Streptavidin is a protein produced by Streptomyces avidinii with strong biotin-binding ability. The non-covalent, yet strong bond between these two molecules has made it a preferable option in biological detection systems. Due to its extensive use, considerable attention is focused on streptavidin production by recombinant methods. Methods: In this study, streptavidin was express...