Introduction: Consumption of vegetable products is increasing commonly in the world because they are recognized as an important source of nutrients, vitamins, and fiber for humans. Salads are among the most widely used foods that are also known as the most contaminated foods in restaurants. This study was conducted to determine the microbial contamination of salad-preparing tables and salad-ser...

introduction: gene expression and purification of luciferases from the firefly, lampyris turkestanicus, and optimization of cellular atp measurements were performed. methods: cdna encoding luciferases from lampyris turkestanicus was transferred from pqe30 vector into pet28a expression vector and pltu28 was built. newly constructed vector was expressed in e. coli xl1 blue and the recombinant luc...

abstract soil contamination with heavy metals is a serious problem in all over the world. soils are contaminated by many different sources of heavy metals. currently different methods are used by researchers for identifying heavy metal contamination. assessing soil metal toxicity with bacterial bioluminescence is one of the new techniques. in this study, different concentrations of zinc and cop...

Background: Leishmania tropica is the cause of more than one form of leishmaniasis and lacks a known reservoir animal. This study compares the potential infectivity of recombinant and wild-type L. tropica in BALB/c mice. Methods: The potential infectivity of recombinant L. tropicaEGFP or L. tropicaEGFP-LUC by two different, the subcutaneous and intradermal, routes was compared using a range of ...

Bioluminescence represents an operational threat to U.S. Navy nighttime operations because of the risk due to flow-stimulated light emission from naturally occurring plankton. Conversely, bioluminescence presents additional capabilities for detecting moving objects at night, particularly in the littoral zone where conventional acoustic surveillance is severely challenged. We are interested in t...

Spectrally resolved bioluminescence optical tomography is an approach to recover images of luciferase activity within a volume using multiwavelength emission data from internal bioluminescence sources. The underlying problem of uniqueness associated with nonspectrally resolved intensity-based bioluminescence tomography is highlighted. Reconstructed images of bioluminescence are presented by usi...

Bioluminescence represents an operational threat to U.S. Navy nighttime operations because of the vulnerability risk due to detection because of flow-stimulated light emission from naturally occurring plankton. Conversely, bioluminescence presents additional capabilities for detecting moving objects at night, particularly in the littoral zone where conventional acoustic surveillance is severely...

Purpose We develop a quantitative bioluminescence assay for in vivo longitudinal monitoring of inflammation in animal models of uveitis. Methods Three models of experimental uveitis were induced in C57BL/6 albino mice: primed mycobacterial uveitis (PMU), endotoxin-induced uveitis (EIU), and experimental autoimmune uveitis (EAU). Intraperitoneal injection of luminol sodium salt, which emits li...

BACKGROUND The light-emitting Vibrios provide excellent material for studying the interaction of cellular communication with growth rate because bioluminescence is a convenient marker for quorum sensing. However, the use of bioluminescence as a marker is complicated because bioluminescence itself may affect growth rate, e.g. by diverting energy. METHODOLOGY/PRINCIPAL FINDINGS The marker effec...