نتایج جستجو برای: biodosimetry

تعداد نتایج: 331  

2016
Chang-Mo Kang Hyun Jin Yun Hanna Kim Cha Soon Kim

Three in vitro dose calibration curves for biodosimetry such as dicentric chromosome assay, fluorescence in situ hybridization (FISH) assay for translocation, and micronuclei (MNs) in binucleated cell assay were established after exposure to ionizing radiation. Peripheral blood lymphocyte samples obtained from healthy donors were irradiated with 60Co source at a dose rate of 0.5 Gy/min to doses...

Journal: :Radiation research 2011
Helen C Turner David J Brenner Youhua Chen Antonella Bertucci Jian Zhang Hongliang Wang Oleksandra V Lyulko Yanping Xu Igor Shuryak Julia Schaefer Nabil Simaan Gerhard Randers-Pehrson Y Lawrence Yao Sally A Amundson Guy Garty

The immunofluorescence-based detection of γ-H2AX is a reliable and sensitive method for quantitatively measuring DNA double-strand breaks (DSBs) in irradiated samples. Since H2AX phosphorylation is highly linear with radiation dose, this well-established biomarker is in current use in radiation biodosimetry. At the Center for High-Throughput Minimally Invasive Radiation Biodosimetry, we have de...

2017
Ruth C. Wilkins Matthew A. Rodrigues Lindsay A. Beaton-Green

Biodosimetry methods, including the dicentric chromosome assay, the cytokinesis-block micronucleus assay and the γH2AX marker of DNA damage are used to determine the dose of ionizing radiation. These techniques are particularly useful when physical dosimetry is absent or questioned. While these assays can be very sensitive and specific, the standard methods need to be adapted to increase sample...

ژورنال: سنجش و ایمنی پرتو 2020

Estimation of absorbed dose for radiation workers or person involved in different radiological accident is the aim of biodosimetry. Cytogenetic methods are the most current and applicable biodosimetry tools. In chronic or protracted exposure, fluorescence in situ hybridization (FISH), stable chromosomal aberration is used for estimation of absorbed dose. For precise estimation of absorbed dose,...

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