The renewable energy sources and plug-in electric vehicles (PEVs) are becoming very popular because of the combination of high fuel costs and concerns about emission issues. This paper presents modelling and control of a Building Integrated Fuel Cell and Plug-in Electric Vehicles (BIFC-PEV) in smart distribution systems. In BIFC-PEV system, conventional building elements could be replaced by sp...

the renewable energy sources and plug-in electric vehicles (pevs) are becoming very popular because of the combination of high fuel costs and concerns about emission issues. this paper presents modelling and control of a building integrated fuel cell and plug-in electric vehicles (bifc-pev) in smart distribution systems. in bifc-pev system, conventional building elements could be replaced by sp...

The simplicity and sensitivity of the bimolecular fluorescence complementation (BiFC) assay make it a powerful tool to investigate protein-protein interactions (PPIs) in living cells. However, non-specific association of the fluorescent protein fragments in a BiFC system can complicate evaluation of PPIs. Here, we introduced a bicistronic expression vector, pBudCE4.1, into an mLumin-based BiFC ...

Bimolecular fluorescence complementation (BiFC) has been widely used in the analysis of protein-protein interactions (PPIs) in recent years. There are many notable advantages of BiFC such as convenience and direct visualization of PPI in cells. However, BiFC has one common limitation: the separated non-fluorescent fragments can be spontaneously self-assembled into an intact protein, which leads...

Over the past decade, bimolecular fluorescence complementation (BiFC) has emerged as a key technique to visualize protein-protein interactions in a variety of model organisms. The BiFC assay is based on reconstitution of an intact fluorescent protein when two complementary non-fluorescent fragments are brought together by a pair of interacting proteins. While the originally reported BiFC method...

Since its introduction in plants 10 years ago, the bimolecular fluorescence complementation (BiFC) method, or split-YFP (yellow fluorescent protein), has gained popularity within the plant biology field as a method to study protein-protein interactions. BiFC is based on the restoration of fluorescence after the two non-fluorescent halves of a fluorescent protein are brought together by a protei...

Bimolecular fluorescence complementation (BiFC) has been widely used to visualize protein-protein interactions (PPIs) in cells. Until now, however, the resolution of BiFC has been limited by the diffraction of light to ∼250 nm, much larger than the nanometer scale at which PPIs occur or are regulated. Cellular imaging at the nanometer scale has recently been realized with single molecule superr...

Plug-in Electric Vehicle (PEV) charging stations couple future transportation systems and power systems. That is, PEV driving and charging behavior will influence the two networks simultaneously. This paper studies optimal planning of PEV fast-charging stations considering the interactions between the transportation and electrical networks. The geographical targeted planning area is a highway t...

Protein networks and signaling cascades are key mechanisms for intra- and intercellular signal transduction. Identifying the interacting partners of a protein can provide vital clues regarding its physiological role. The bimolecular fluorescence complementation (BiFC) assay has become a routine tool for in vivo analysis of protein-protein interactions and their subcellular location. Although th...

Pulmonary extravascular fluid volume (PEV) has been estimated by using a double isotope technique in nine normal subjects and 45 patients with valvular heart disease. The method was reproducible, the standard deviation being + 15.2 ml/m2, or 11.2% of the mean. Groups of patients with aortic and mitral valve disease had significantly higher values of PEV than normal persons; some patients with p...