A sensitive RIA for benzoylecgonine in body fluids is described. The label is radioiodinated p-hydroxybenzoylecgonine. The antiserum from an Emit-dau cocaine metabolite kit (Syva) is used at a 1:600 dilution. The standards are benzoylecgonine in buffer. PEG-6000 is used to separate the bound and free fractions. Cocaine, ecgonine, and methylecgonine have cross-reactivities of 2%, 1%, and 0.1%, r...

To accurately assess the mechanism of involvement of the active metabolite norcocaine in the effects of oral cocaine, it is essential to determine the rate and extent of the formation of norcocaine. Although this study was designed specifically for this aim, it was also of interest to characterize the metabolite kinetics of benzoylecgonine for comparative purpose. We first characterized the pha...

In order to support studies on various medication protocols for the treatment of cocaine abuse, an accurate, precise, and sensitive (2.5 to 750 ng/mL) liquid chromatography-tandem mass spectrometry assay was developed to determine cocaine and benzoylecgonine in human plasma. Cocaine-d3 and benzoylecgonine-d3 were added as internal standards and samples were subjected to solid-phase extraction. ...

A quantitative analytical procedure for the determination of cocaine, benzoylecgonine, cocaethylene, and norcocaine in hair has been developed and validated. The hair samples were washed, incubated, and any drugs present were quantified using mixed-mode solid-phase extraction and liquid chromatography with tandem mass spectrometric detection in positive atmospheric pressure chemical ionization ...

Carboxylesterases are important in the metabolism of cocaine, catalyzing the hydrolysis of cocaine to its two major metabolites, benzoylecgonine and ecgonine methyl ester. In the presence of ethanol, some cocaine undergoes transesterification with ethanol instead of hydrolysis with water producing the active metabolite, cocaethylene. The metabolic fate of cocaethylene is unknown, but given its ...

The aim of this study was to evaluate the extend passive exposure in hair from professionals working contact with cocaine seizures, considering cleaning procedures, segmentation and interpretation recommendations consensus on drug testing [https://soht.org/images/pdf/Consensus_DoA_2021.pdf] recent literature. In forensic casework where analysis is requested, experts have answer question if conc...

We evaluated 72 clinical Neisseria gonorrhoeae isolates for in vitro susceptibility to cocaine hydrochloride and its metabolite benzoylecgonine and to penicillin, tetracycline, erythromycin, ceftriaxone, and ofloxacin. Although there was a wide range of susceptibilities to the antimicrobial agents, cocaine and its major metabolite, benzoylecgonine, had no demonstrable antigonococcal activity. C...

Using the enzyme immunoassay technique (EMIT), we determined the time course of urinary excretion of benzoylecgonine in 16 surgical patients and three volunteers who received intranasal cocaine. After doses varying from 13 to 130 mg, the test for benzoylecgonine was positive in 1 to 4 h, peaked at about 10 to 12 h, remained positive for 18 to 27 h, and became negative after about 17 h. This inf...

Cocaine is a common drug of abuse. To detect its use, a screening detection concentration for the cocaine metabolite benzoylecgonine is commonly set at 150 ng/mL and its confirmatory cut-off is set at 100 ng/mL. Studies have suggested that these cut-offs may be set too high, allowing some patients with this substance abuse problem to be missed or improperly monitored. With the advent of liquid ...