Halotolerant Bacillus aquimaris VITP4, isolated from Kumta coast (India), was used to produce extracellular α-amylase. The production was found to be maximal after 24 h of growth at pH 8.0 and 40 C. Optimal activity of the purified enzyme was in the pH range of 7.5 – 9.5 at 40 C. The enzyme was found to retain more than 60% of the initial activity even at NaCl concentration of 3.5 M, indicating...

Background: It was previously shown that the activity of a serine protease from a moderately halotolerant Bacillus aquimaris VITP4 strain is active in a wide range of pH and temperatures and could be modulated by the presence of the divalent metal ions.  Objectives: In the present study, a quantitative analysis was done in order to explore the parameters that are contributing to the protease a...

Bacillus aquimaris TF12 is a Gram-positive bacteria isolated from a tidal flat of the Yellow Sea in South Korea. We report the draft whole-genome sequence of Bacillus aquimaris TF12, the type strain of a set of bacteria typically associated with marine habitats and with a potentially high biotechnology value.

A halotolerant bacterium Bacillus acquimaris VITP4 was used for the production of extracellular protease. Fractional precipitation using ammonium chloride was used to obtain the enzyme. The protease exhibited optimum activity at pH 8.0 and 40 degrees C and retained 50% of its optimal proteolytic activity even in the presence of 4 M NaCl, suggesting that it is halotolerant. The molecular mass of...

0.094 ----TSB 37°C Baciillus sp. Clone 35 23 0.047 ----TSB 37°C Bacillaceae bacterium C22 21 1.7 ----BA 37°C Bacillus cereus 43 0.75 ----BA 30°C 38 0.5 ----TSB 30°C Bacillus fusiformis 14 1.5 ----BA 37°C Bacillus aquimaris 45 1.2 CXM30 4.25mm BA 30°C Bacillus pumilus 48 3.5 ----R2A 37°C Bacillus megaterium 32 0.064 ----BA 30°C 42 0.75 ----TSB 37°C 19 0.25 ----TSB 30°C 18 0.38 CXM30 1.75mm LB 37°C

Bacillus aquimaris strain SAMM, a biosurfactant producing moderately halophilic marine bacterium was isolated from Indian Arabian coastline sea water. The strain was found to tolerate up to 2.7 M NaCl indicating osmotic stress sustainable physiological systems. We report here the draft genome sequence of B. aquimaris SAMM, as a candidate bacterium for bioactive surfactant producer. The whole ge...

The α-amylases from Anoxybacillus species (ASKA and ADTA), Bacillus aquimaris (BaqA) and Geobacillus thermoleovorans (GTA, Pizzo and GtamyII) were proposed as a novel group of the α-amylase family GH13. An ASKA yielding a high percentage of maltose upon its reaction on starch was chosen as a model to study the residues responsible for the biochemical properties. Four residues from conserved seq...

A moderately halophilic, Gram-positive and rod-shaped bacterium, strain m4-4T, was isolated from a Chihuahuan desert lagoon in Cuatro Ciénegas, Coahuila, Mexico. Strain m4-4T was found to grow optimally at 30-37 degrees C, pH 7.0-8.0 and 5 % NaCl and to tolerate from 0.5 % to 10 % NaCl. It was shown to be aerobic. The genomic DNA G+C content was about 37 mol%. Strain m4-4T exhibited minimal or ...

A halotolerant, round-endospore-forming, aerobic, Gram-positive bacterium, designated BH724(T), was isolated from a solar saltern at Taean in Korea. Cells of this strain were rod-shaped and found to be non-motile. Strain BH724(T) grew at salinities of 0-10 % (w/v) NaCl with an optimum of 3 % (w/v) NaCl and at temperatures of 15-50 degrees C with an optimum of 40 degrees C. Phylogenetic analyses...