With the goal of creating faster and more efficient methods of generating unique Ac/Ds insertional mutants in a population of Fragaria vesca, various methods of seed germination, kanamycin screening, and the effects of varying nitrogen fertilization on diploid strawberry have been examined. Seed germination was improved to 42% in B5 liquid medium compared to _ on MS solid medium. Kanamycin scre...

Crowell, Richard L. (Hahnemann Medical College, Philadelphia, Pa.). Specific viral interference in HeLa cell cultures chronically infected with Coxsackie B5 virus. J. Bacteriol. 86:517-526. 1963.-The presence of large amounts of Coxsackie B5 virus in culture fluids of a HeLa subline, serially propagated over a 3-year period, provided evidence for attainment of a viral carrier state. Human serum...

one of the important methods of plant propagation in large scale so as genetic manipulation and artificial seed production is somatic embryogenesis technique. the obtained results from this methods related to different factors including condition of culture medium. therefore, the aim of this study conduction were determining the role of molybdenum during carrot petiole somatic embryogenesis. at...

The effect of abscisic acid (ABA) and excess benzyladenine (BA) on the formation of shoot from tea (Camellia sinensis L. assamica × sinensis) leaf was investigated in this research. Callus was formed and grew well when explants were cultured on LS basal medium supplemented with (in mg/L) thiamine-HCl, 1.25; pyridoxine-HCl, 0.625; nicotinic acid, 0.625; indole-3-acetic acid (IAA), 30; naphthalen...

∗ Corresponding author Root cultures of Plumbago rosea Linn. were established from young leaf explants on solid Gamborg's B5 (B5) medium supplemented with the combination of αnaphthalene acetic acid (NAA) and kinetin in the concentration ranges of 0.5-2.0 mg/l and 0.1-0.5 mg/l, respectively. The production of plumbagin, determined by TLC-densitometry was higher [0.016 ± 0.0030% dry weight (DW)]...

Lisianthus (Eustoma grandiflorum L.) is a very popular, ornamental plant. Due to low germination of its seeds, usual techniques for its propagation are not efficient. The present study was aimed to establish callus from explants of different plant parts of Eustoma grandiflorum L. Raf Shinners in B5, LS and MS media with modifications.  The results showed that among different plant parts, leaf e...

Micropropagation is an effective way to preserve the gene pool of threatened plants. This study devoted mathematical modeling nutrient media and effect mT (meta-topoline) on multiplication shoots Hedysarum grandiflorum, Hyssopus cretaceus, Matthiola fragrans in vitro comparison with benzylaminopurine (BAP) kinetin (KT). Initiation was performed MS medium 0.5 mg/L BAP. For multiplication, MS, B5...

So far there is no information about receiving red beet androgenic embryos by androgenesis. Several factors were tested which affected this process: starch accumulation in microspores, correlation between bud length and microsporogenesis course, induction and regeneration medium composition. Ploidy level of obtained regenerants were evaluated. Treating anthers with α-amylase or watering donor p...

In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins BAP and KIN...

An efficient method has been developed for large-scale multiplication of Curculigo orchioides (Hypoxidaceae), an endangered medicinal plant, through direct bulbil formation from leaf explants in shake flask cultures. Leaf-segments (7 x 10 mm) were cultured in B5 liquid medium containing KNO3 (200 mgNL-1), (NH4)2SO4 (50 mgNL-1), benzyl adenosine (2.2 microM), adenine (0.11 mM), indole butyric ac...