Protein-protein interactions are inherently anisotropic to some degree, with orientation-dependent interactions between repulsive and attractive or complementary regions or "patches" on adjacent proteins. In some cases it has been suggested that such patch-patch interactions dominate the thermodynamics of dilute protein solutions, as captured by the osmotic second virial coefficient (B22), but ...

The human leukocyte antigen (HLA)-B22 serogroup--which consists of the alleles B*54, B*55, and B*56--has been associated with rapidly progressive disease in white patients with human immunodeficiency virus (HIV) infection. Subjects from 3 cohorts of men who have sex with men (N=671), all of whom experienced HIV-1 seroconversion at roughly the same time, were molecularly typed at HLA-A, -B, and ...

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Studies on monomeric insulin with reduced self-association are important in the development of insulin pharmaceutical preparations with rapid hypoglycemic action on patients with diabetes. Here we report a novel monomeric insulin, B22 Glu des-B30 insulin, prepared from a single chain insulin precursor with B22 Arg mutated to Glu, which was expressed in Pichia pastoris and converted to B22 Glu d...

T4 bacteriophage mutant am B22 (gene 43), an amber mutant in the gene for DNA polymerase, produces a protein which lacks the polymerase but retains the nuclease function of the wild type enzyme. The am B22 nuclease is shown to be a fragment of the wild type polymerase by comparing the tryptic peptides of the two proteins. The wild type and mutant enzymes each consist of a single polypeptide cha...

As cerebral toxoplasmosis is the most common cerebral focal lesion in AIDS patients, this study evaluated three PCR markers for diagnosis, since some limitations remain present, such as low parasite levels in some clinical samples. The molecular markers were B22-B23 and Tg1-Tg2 (based on the B1 gene) and Tox4-Tox5 (non-coding fragment, repeated 200-300-fold). DNA samples from 102 AIDS patients ...

This paper presents performance study of Peugeot MM540 diesel engine using biodiesel prepared from used cooking oil. Transesterification process is followed to produce bio diesel and is blended with diesel in different proportion of 22:78 and 24:76 by volume and named as blend B22 and B24 respectively. Engine was run at different loads (0, 15, 30, 35, and 40kg) with constant speed of 1000rpm se...

Schistosomiasis is a major parasitic disease of humans, second only to malaria in its global impact. The disease is caused by digenean trematodes that infest the vasculature of their human hosts. These flukes are limited externally by a body wall composed of a syncytial epithelium, the apical surface membrane of which is a parasitism-adapted dual membrane complex. Annexins are thought to be of ...