Ephedra sinica polysaccharides have been reported to possess important activities, so quality evaluation of polysaccharides from the genus Ephedra is urgent. In this study, enzymatic digestions were performed to establish multiple saccharide fingerprints by ultra-performance liquid chromatography with electrospray ionization triple quadrupole linear ion trap mass spectrometry (UPLC-ESI-TQ-MS/MS...

the spermatogonial stem cells (sscs) are founder of spermatogenesis and they are the only stem cells that can transmit genetic materials to offspring’s. this research was conducted to achieving a reliable origin of sscs from the goat testicular tissues according to a four steps procedure; a) collection and preparing testicular tissues, b) two enzymatic digestions, c) using mesh and cell suspens...

The complete amino acid sequences of [2Fe-2S] ferredoxins from Scopolia japonica and Lycium chinense have been determined by automated Edman degradation of the entire Cm-proteins and of the peptides obtained by enzymatic digestions. These two ferredoxins exhibited only 2-7 differences in the amino acid sequence when compared to the Datura-ferredoxins (D. stramonium, D. metel, and D. arborea), a...

The intermediate hosts of Schistosoma mansoni, in Brazil, Biomphalaria glabrata, B. tenagophila and B. straminea, were identified by restriction fragment length polymorphism analysis of the mitochondrial gene cytochrome oxidase I (COI). We performed digestions with two enzymes (AluI and RsaI), previously selected, based on sequences available in Genbank. The profiles obtained with RsaI showed t...

The purpose of this study was to develop a smart proteolysis sampler based on commercially available VAMS® tips employing Volumetric Absorptive Microsampling. In proof-of-concept paper we show that covalently bound trypsin VAMS exhibits sufficient activity for proteomic purposes without altering physical conditions. Alkaline pH needed the covalent binding and at least 50 μg required obtain maxi...

The fine structure of desmosomes is well established (1-3), and some information about their chemical composition is available (4-6) . Recently techniques were developed for performing enzymatic digestions on osmium tetroxide-fixed, alcoholor acetone-dehydrated and epoxyembedded tissues (7-10) . These methods can determine with considerable specificity the organic components of many cell struct...

oligosaccharide prepared from Pl.-B was eluted as a single peak on both reversed-phase {RP-) HPLC and size-fractionation (SF-) HPLC, and its strueture was estimated to be Manoct-qManal -. 3)(Xylfi1-.2) Manfi1-4GlcNAcfi1-4(Fuc=1-3)GlcNAc by a combination of component analysis, successive exogl)'cosidase digestions, IS-MS anal)'sis, and 500MHz 'H-NMR, Two tryptic gl)'copeptides were isolated from...

oligosaccharide prepared from Pl.-B was eluted as a single peak on both reversed-phase {RP-) HPLC and size-fractionation (SF-) HPLC, and its strueture was estimated to be Manoct-qManal -. 3)(Xylfi1-.2) Manfi1-4GlcNAcfi1-4(Fuc=1-3)GlcNAc by a combination of component analysis, successive exogl)'cosidase digestions, IS-MS anal)'sis, and 500MHz 'H-NMR, Two tryptic gl)'copeptides were isolated from...

The use of substrates containing well defined adducts at precise sites, is required to perform a careful analysis of the toxic and mutagenic potential of a lesion. As a first step in this direction the octamer 5'-d(CCGGCGGT), containing the sequence of the codons 12 d(GGC) and 13 d(GGT) of the human H-ras gene, was reacted with the antitumoral drug cis-diamminedichloroplatinum(II). The platinat...