Strains of bacteria in axenic culture were isolated from samples of depths between 1,957 and 10,476 m of the Pacific Ocean. All of the bacteria from this range of depths were barophilic. The pressure at which the rate of reproduction was maximal was found to be correlated with the depth of origin of the isolates.

In Caenorhabditis elegans, several manipulations that affect nutrition slow development, reduce fecundity, and increase life span. These are viewed as dietary restriction (DR) and include culture in semidefined, nutrient-rich liquid medium that is axenic (i.e., there is no microbial food source). Here we describe convenient ways to exert DR by culture on agar plates containing axenic medium. We...

background: promastigotes of leishmania spp. have been readily cultured, but the axenic culture of amastigotes has been successful in l. donovani , l .infantum l. mexicana and l. pifanoi . however, some species such as l. major, is much less amenable to axenic cultivation. in present study, we describe an in vitro culture system for the generation and propagation of axenic amastigotes form of l...

Axenic cultures of free-living aerobic ciliates, such as Tetrahymena thermophila and Paramecium aurelia, have been established and routinely used in laboratory research, greatly facilitating, or enabling characterization of their metabolism, physiology, and ecology. Ruminal protozoa are anaerobic ciliates, and they play important roles in feed digestion and fermentation. Although, repeatedly at...

We sequenced and annotated genomes of two haloalkaliphilic Deltaproteobacteria, Geoalkalibacter ferrihydriticus Z-0531(T) (DSM 17813) and Geoalkalibacter subterraneus Red1(T) (DSM 23483). During assembly, we discovered that the DSMZ stock culture of G. subterraneus was contaminated. We reisolated G. subterraneus in axenic culture and redeposited it in DSMZ and JCM.

background: the main goal of the present study was to set up an axenic cultivation of acanthamoeba and assess the pathogenic ability of t4 genotypes from different clinical and environmental strains of acanthamoeba using two physical assays. methods: sixteen acanthamoeba isolates including 10 environmental and 6 clinical strains were cul-tured axenically. axenic cultivation was performed using ...

We evaluated the contribution of amoebic coculture to the recovery of Legionella spp. from 379 respiratory samples. The sensitivity of axenic culture was 42.1%. The combination of axenic culture with amoebic coculture increased the Legionella isolation rate to 47.1%. Amoebic coculture was particularly efficient in isolating Legionella spp. from respiratory samples contaminated with oropharyngea...

A new artificial nutritive medium has been developed for the axenic culture of Artemia enabling the production of adults in less than 1 wk. The techniques for its preparation have been detailed. Its utilization is recommended for the standardized production of experimental animals.