background: the main goal of the present study was to set up an axenic cultivation of acanthamoeba and assess the pathogenic ability of t4 genotypes from different clinical and environmental strains of acanthamoeba using two physical assays. methods: sixteen acanthamoeba isolates including 10 environmental and 6 clinical strains were cul-tured axenically. axenic cultivation was performed using ...

Continuous axenic culture of Pneumocystis carinii has been achieved. A culture vessel is used that allows for frequent medium exchange without disturbance of organisms that grow attached to a collagen-coated porous membrane. The growth medium is based on Minimal Essential Medium with Earle's salt supplemented with S-adenosyl-L-methionine, putrescine, ferric pyrophosphate, N-acetyl glucosamine, ...

BACKGROUND The main goal of the present study was to set up an axenic cultivation of Acanthamoeba and assess the pathogenic ability of T4 genotypes from different clinical and environmental strains of Acanthamoeba using two physical assays. METHODS Sixteen Acanthamoeba isolates including 10 environmental and 6 clinical strains were cultured axenically. Axenic cultivation was performed using P...

Marine-derived bacteria and fungi are promising sources of novel bioactive compounds that are important for drug discovery programs. However, as encountered in terrestrial microorganisms there is a high rate of redundancy that results in the frequent re-discovery of known compounds. Apparently only a part of the biosynthetic genes that are harbored by fungi and bacteria are transcribed under ro...

background: promastigotes of leishmania spp. have been readily cultured, but the axenic culture of amastigotes has been successful in l. donovani , l .infantum l. mexicana and l. pifanoi . however, some species such as l. major, is much less amenable to axenic cultivation. in present study, we describe an in vitro culture system for the generation and propagation of axenic amastigotes form of l...

The frequent re-isolation of known compounds is one of the major challenges in drug discovery. Many biosynthetic genes are not expressed under standard culture conditions, thus limiting the chemical diversity of microbial compounds that can be obtained through fermentation. On the other hand, the competition during co-cultivation of two or more different microorganisms in most cases leads to an...