abstract background: for infertile women aged over 35 years, failure of the zp (zona pellucida) to rupture is believed to be associated with a decreased implantation rate in in vitro fertilization (ivf) or intra cytoplasmic sperm injection (icsi). objective: in this research, laser assisted hatching (lah) was offered to patients with advanced maternal age to evaluate a possible benefit. materia...

objective: the aim of this study was to investigate the effect of laser assisted hatching on the development and quality of vitrified-warmed 4-cell stage mouse embryos. materials and methods: the vitrified-warmed 4-cell mouse embryos were divided into two groups: control group (without laser assisted hatching) and experiment group (with laser assisted hatching). all embryos in both groups were ...

background: routine use of assisted hatching (ah) following icsi is a controversial issue in the literature. there are rare studies regarding the effect of laser assisted hatching (lah) on live birth rate. objective: our main goal was to evaluate the effect of lah on delivery rate as well as congenital anomaly in patients undergoing their first icsi cycle. materials and methods: a total of 182 ...

OBJECTIVE In search of an ideal method of assisted hatching (AH), we compared the effects of conventional micropipette-AH and laser-AH on the blastocyst formation rate (BFR) and blastocyst cell numbers. METHODS Four- to five-week-old ICR female mice were paired with male mice after superovulation using Pregnant mare's serum gonadotropin (PMSG) and hCG. The two-cell embryos were flushed from t...

BACKGROUND Focus on the hatching process has so far been in the field of fresh embryos. Cryopreserved-thawed embryos have a lower rate of pregnancy than fresh embryos, which might be due to hardening of the zona pellucida. METHODS During a 2 year period, a prospective randomized study enrolling 253 cryopreserved-thawed cycles was performed on day 2 embryos. Pseudorandomization to assisted hat...

OBJECTIVE To compare the clinical outcomes of patients with vitrified-thawed embryos transferred using either the 0.25 mL straw method and the pull and cut straw (PNC) method. To evaluate the clinical outcomes of patients with transferred embryos that underwent assisted hatching at the cleaved embryo (day 3) or the blastocyst (day 5) stage. METHODS The study population consisted of women who ...