OBJECTIVE: The aim of this study was to investigate the effect of pomegranate juice (PJ) on the paraoxonase and arylesterase activity of human serum paraoxonase( PON1). MATERIAL AND METHOD: Fifty patients with type 2 diabetes mellitus consumed 200 ml of PJ daily for a period of 6 weeks. Blood was collected from the patients before and after PJ consumption after 12 h of fasting. Blood sugar, to...

objective: the aim of this study was to investigate the effect of pomegranate juice (pj) on the paraoxonase and arylesterase activity of human serum paraoxonase( pon1). material and method: fifty patients with type 2 diabetes mellitus consumed 200 ml of pj daily for a period of 6 weeks. blood was collected from the patients before and after pj consumption after 12 h of fasting. blood sugar, tot...

Paraoxonase-1 (PON1) is known to be associated with high density lipoproteins. We optimized buffer conditions to obtain quantitative recovery of PON1 (arylesterase) activity and analyzed the distribution of PON1 in mice using a combination of size-exclusion chromatography and ultracentrifugation. Size-exclusion chromatography of mouse serum separated the esterase activity into two peaks, one ov...

A new method for phenotyping human serum arylesterase (EC 3.1.1.2) is described and evaluated. The aromatic esters, phenyl acetate and 4-nitrophenyl acetate, were compared as substrates for spectrophotometric measurement of arylesterase activity. A method for arylesterase phenotyping, based upon inhibition of the enzymatic hydrolysis of 4-nitrophenyl acetate by phenyl acetate, was developed. Th...

CONTEXT Cholinesterase (ChE) specific activity is the ratio of ChE activity to ChE mass and, as a biomarker of exposure to cholinesterase inhibitors, has a potential advantage over simple ChE activity. OBJECTIVE To examine the association of several potential correlates (serum arylesterase/paraoxonase activity, serum albumin, sex, age, month of blood collection, and smoking) with plasma ChE s...

human serum paraoxonase (pon) associated with high density lipoprotein (hdl) , has been postulated to have a role in protecting low-density lipoprotein (ldl) against oxidative damage, which has led to the proposal that pon is an anti-atherogenic and anti-inflammatory enzyme. it has genetic polymorphism at the 191 (r ->q) and two alloenzymes a and b and three phenotypes a, b, and ab. we examined...

oxidative stress is involved in the pathogenesis of asthma. paraoxonase 1 (pon1) and arylesterase are esterase enzymes displaying antioxidant characteristics. pon1 activity varies widely among individuals and ethnic groups, partly related to polymorphisms. the aim of this study was to determine the activities of pon1 and arylesterase including the phenotype distribution of pon1 in asthmatic pat...

Human paraoxonase (PON1) exists in 2 major polymorphic forms and has been shown to protect LDL and HDL against oxidation. The aim of this study was to assess the differences between subjects at increased risk of cardiovascular disease (CVD), taking into account the effects of PON1-Q192R and PON1-L55M polymorphisms on 1) basal serum arylesterase activity, lipid peroxidation (LPO), and LDL-choles...

BACKGROUND Diminished serum arylesterase activity, catalyzed by the high-density lipoprotein-associated paraoxonase-1, is associated with heightened systemic oxidative stress and atherosclerosis risk. In the present study, we sought to determine the prognostic role of serum arylesterase activity in subjects with systolic heart failure, particularly in relation to established cardiac biomarkers....