نتایج جستجو برای: aroa mutant

تعداد نتایج: 129782  

A. R. Jabbari, G. R. Moazzeni Jula M. Esmailzadeh M. Tabatabaei,

Haemorrhagic septicaemia (HS) is a fatal systemic disease of cattle and buffaloes. Some control isachieved with administration of alum-precipitated or oil-adjuvanted killed whole-cell vaccines injectedsubcutaneously. These vaccines, however, provide only short-term immunity and for effective use, theyshould be administered annually. We constructed an aroA attenuated derivative of a Pasteurella ...

Journal: :iranian journal of veterinary research 2007
m. tabatabaei g. r. moazzeni jula a. r. jabbari m. esmailzadeh

haemorrhagic septicaemia (hs) is a fatal systemic disease of cattle and buffaloes. some control isachieved with administration of alum-precipitated or oil-adjuvanted killed whole-cell vaccines injectedsubcutaneously. these vaccines, however, provide only short-term immunity and for effective use, theyshould be administered annually. we constructed an aroa attenuated derivative of a pasteurella ...

Journal: :Journal of general microbiology 1993
C O'Connell P A Pattee T J Foster

The aroA gene of Staphylococcus aureus 8325-4 was cloned. Sequence analysis and the phenotype of directed plasmid insertions 5' to aroA suggest that aroA is located in an operon and that it maps 3' to the aroC and aroB genes. A revised consensus sequence for the aroA gene product EPSP synthase binding site for its substrate (phosphoenolpyruvate) and an inhibitor (glyphosate) is proposed. An aro...

Journal: :Infection and immunity 2006
Fernanda R Buzzola María Sol Barbagelata Roberto L Caccuri Daniel O Sordelli

Staphylococcus aureus is the most important etiological agent of bovine mastitis, a disease that causes significant economic losses to the dairy industry. Several vaccines to prevent the disease have been tested, with limited success. The aim of this study was to obtain a suitable attenuated aro mutant of S. aureus by transposon mutagenesis and to demonstrate its efficacy as a live vaccine to i...

Journal: :Microbiology 1996
P C Oyston P Russell E D Williamson R W Titball

This study describes a PCR-based approach for the production of a rationally attenuated mutant of Yersinia pestis. Degenerate primers were used to amplify a fragment encoding 91.45% of the aroA gene of Y. pestis MP6 which was cloned into pUC18. The remainder of the gene was isolated by inverse PCR. The gene was sequenced and a restriction map was generated. The Y. pestis aroA gene had 75.9% ide...

Journal: :Applied and environmental microbiology 2004
José Vivas Begoña Carracedo Jorge Riaño Blanca E Razquin Pilar López-Fierro Félix Acosta Germán Naharro Alberto J Villena

Genetically modified auxotrophic mutants of different fish pathogens have been used as live vaccines in laboratory experiments, but the behavior of the strains after release into aquatic ecosystems has not been characterized. We previously constructed and characterized an aroA mutant of Aeromonas hydrophila and studied the protection afforded by this mutant as a live vaccine in rainbow trout. I...

Journal: :Infection and immunity 1998
C Hernanz Moral E Flaño del Castillo P López Fierro A Villena Cortés J Anguita Castillo A Cascón Soriano M Sánchez Salazar B Razquín Peralta G Naharro Carrasco

The aroA gene of Aeromonas hydrophila SO2/2, encoding 5-enolpyruvylshikimate 3-phosphate synthase, was cloned by complementation of the aroA mutation in Escherichia coli K-12 strain AB2829, and the nucleotide sequence was determined. The nucleotide sequence of the A. hydrophila aroA gene encoded a protein of 440 amino acids which showed a high degree of homology to other bacterial AroA proteins...

2012
Taghi Zahraei Salehi Saeid Tabatabaei Vahid Karimi Bahar Nayeri Fasaei Abdullah Derakhshandeh Ali Omid Nekoui Jahromi

Colibacillosis is an important disease in the poultry industry which causes serious economic damages. As it is suggested that vaccination is one of the means to control colibacillosis, we tried to investigate the vaccine potential of a ∆aroA derivative of an O78:K80 avian pathogenic Escherichia coli containing increased serum survival gene. 490 chicks were selected as follows: For assessment of...

Journal: :Infection and immunity 2002
Gregory P Priebe Mary M Brinig Kazue Hatano Martha Grout Fadie T Coleman Gerald B Pier Joanna B Goldberg

Antibodies to the lipopolysaccharide O antigen of Pseudomonas aeruginosa mediate high-level immunity, but protective epitopes have proven to be poorly immunogenic, while nonprotective or minimally protective O-antigen epitopes often elicit the best immune responses. With the goal of developing a broadly protective P. aeruginosa vaccine, we used a gene replacement system based on the Flp recombi...

Journal: :Gene 1996
M M Wösten H J Dubbink B A van der Zeijst

The gene for 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase (aroA) cloned from Campylobacter jejuni (Cj) strain 81116 was identified by complementation of an Escherichia coli (Ec) auxotrophic aroA mutant. The Cj aroA gene has been sequenced. It encodes an enzyme of 428 amino acids (aa), that is homologous to other bacterial EPSP synthases, especially that of Bacillus subtilis with which it ...

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