Armillaria mellea was once thought to be a morphologically variable species with a wide distribution and a very broad host range. The relatively recent development of an indirect assessment of sexual incompatibility in Armillaria has led to identification of biological species within A. mellea s. 1. Partial intergenic spacer region (IGS) sequences of the ribosomal RNA (rRNA) operon have been de...

Polyporus umbellatus, a species symbiotic with Armillaria mellea and it also exhibits substantial defence response to Armillaria mellea infection. There are no genomics resources databases for understanding the molecular mechanism underlying the infection stress of P. umbellatus. Therefore, we performed a large-scale transcriptome sequencing of this fungus with A. mellea infection using Illumin...

Armillaria root disease is a significant forest health concern in the Atlantic forest ecosystems in Spain. The damage occurs in conifers and hardwoods, causing especially high mortality in young trees in both native forests and plantations. In the present study, the distribution of Armillaria root disease in the forests and plantations of the Basque Country is reported. Armillaria spp. were mor...

Armillaria mellea is a significant pathogen that causes Armillaria root disease on numerous hosts in forests, gardens and agricultural environments worldwide. Using a yeast-adapted pCAMBIA0380 Agrobacterium vector, we have constructed a series of vectors for transformation of A. mellea, assembled using yeast-based recombination methods. These have been designed to allow easy exchange of promote...

During May 2010, sporocarps of what appeared to be an Armillaria sp. were found in large clumps in historic Kirstenbosch Botanical Gardens on the foot of Table Mountain, Cape Town, South Africa. These sporocarps could be physically linked to the roots of unidentified dead trees and Protea spp. The aim of this study was to identify the Armillaria sp. found fruiting in Kirstenbosch. To achieve th...

We isolated and characterized 12 microsatellite markers for two North American populations (California, Pennsylvania) of Armillaria mellea, a fungal pathogen responsible for Armillaria root disease of numerous woody plants. Allele frequency ranged from two to nine alleles per locus, and gene diversity ranged from 0.05 to 0.86. Of the 12 loci, eight loci were polymorphic in the California and Pe...

To produce an artificial fruiting body of Armillaria mellea on the oak sawdust medium, seven strains of A. mellea were used. The top surface of oak sawdust medium covered with ground raw carrot was inoculated with each of 7 strains and cultured for 30 days at 25℃ in the dark condition until the mycelia of A. mellea completely colonized the medium from top to bottom. Then, the mycelia which were...

Armillaria mellea and Lycoperdon saccatum are two delicious mushrooms growing widely trough all Balkan region. Investigation of A. mellea and L. saccatum antioxidant properties includes preparation of mushrooms extracts, determination of Selenium and Zinc content and evaluation of theirs antioxidant activity involving scavenging activity of ̇O2 radicals, DPPH and reducing power assay. Higher ext...

UNLABELLED Armillaria mellea, known as honey mushroom, has been used both as food and medicine. AIM In this work, the ethanolic and hydromethanolic extracts from Armillaria mellea fruiting bodies were investigated for their phenolic content, antioxidant and antihyperglycemic effects (in vitro studies). MATERIAL AND METHODS The total phenolics were quantified using Folin-Ciocalteu assay. The...

Antioxidant components, ascorbic acid, total flavonoids and total phenols are produced effectively by Armillaria mellea submerged cultures. Dried mycelia and mycelia-free broths obtained by A. mellea submerged cultures are extracted with methanol and hot water and investigated for antioxidant properties. Methanolic extracts from dried mycelia (MEM) and mycelia-free broth (MEB) and hot water ext...