background and purpose: cadmium is hazardous and non-biodegradable material entering the food chain. in this paper, the removal of cadmium from aqueous solutions by sunflower powder (the natural biosorbent) was investigated. materials and methods: the experiments were performed in a batch system. the effect of parameters such as contact time, ph, cadmium concentration and adsorbent dose were ev...

ger G and Albertsson PA (1983) AfRnity partitioning of phosphofructokinase from baker’s yeast using polymer-bound Cibacron blue F3GA. European Journal of Biochemistry 131: 589}594. KopperschlaK ger G and Birkenmeier G (1990) AfRnity partitioning and extraction of proteins. Bioseparation 1: 235}254. Tjerneld F, Johansson G and Joelsson M (1987) AfRnity liquid}liquid extraction of lactate dehydro...

PURPOSE To determine whether myocilin (MYOC; also referred to as TIGR) is present as a complex in human aqueous humor, whether part of the complex formation may be due to MYOC-MYOC interactions and to characterize the sites of interaction. METHODS Human aqueous humor was analyzed by using a gel filtration column for the identification of MYOC complexes. MYOC-MYOC interactions were studied wit...

aqueous two-phase systems (atps) have  emerged as a powerful extraction method for the downstream processing of bio-molecules. the aim of this work was to investigate the possibility of utilizing atps for the separation of recombinant bacillus sphaericus phenylalanine dehydrogenase (phedh). polyethylene glycol (peg) and ammonium sulfate systems were selected for our experiment. the effect of di...

Cephalexin is an important antibiotic. It is very significant to determine an appropriate method for the extraction of this valuable antibiotic for industrial applications. Aiming at developing an efficient method for the extraction of cephalexin, the partitioning of cephalexin has been evaluated in aqueous two-phase system, including cholinium chloride and potassium phosphate. The effect of th...

background: phenylalanine dehydrogenase (phedh ec 1.4.1.20) is a nad+-dependent enzyme that performs the reversible oxidative deamination of l-phenylalanine to phenylpyruvate. it plays an important role in detection and screening of phenylketonuria (pku) diseases and production of chiral intermediates as well. the main goal of this study was to find a simple and rapid alternative method for pur...

Abstract Aqueous two-phase extraction is an ecologically safe method for heavy metal recovery and regeneration from contaminated aqueous solutions. Investigation has been carried out experimentally the of lead solutions using Polyethylene Glycol 1500-Ammonium sulfate system. The extracting agent used sodium chloride. experiments Pb(II) have performed in batch mode according to central composite...