نتایج جستجو برای: apobec3g
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سابقه و هدف: مولکول APOBEC3G ، به عنوان فاکتور سلولی دخیل در میانکنش با vif HIV-1 عمل میکند که برای بررسی میانکنش آن با vif HIV-1 ، از سلولهای ناپذیرای طبیعی و ذاتی مثل H9 و PBMC انسانی مولد APOBEC3G استفاده میشود. به دلیل ناکارآمدی آنها در این نوع بررسیها، نیازمند سلولهای ناپذیرایی بودیم که به طور ثابت و به میزان بالا تولید APOBEC3G بنماید. لذا از سلولهای 293T برای ایجاد این دسته از سلول...
APOBEC3G and APOBEC3F are cytidine deaminase with duplicative cytidine deaminase motifs that restrict HIV-1 replication by catalyzing C-to-U transitions on nascent viral cDNA. Despite 60% protein sequence similarity, APOBEC3F and APOBEC3G have a different target consensus sequence for editing, and importantly, APOBEC3G has 10-fold higher anti-HIV activity than APOBEC3F. Thus, APOBEC3F and APOBE...
سابقه و هدف: مولکول apobec3g ، به عنوان فاکتور سلولی دخیل در میانکنش با vif hiv-1 عمل میکند که برای بررسی میانکنش آن با vif hiv-1 ، از سلولهای ناپذیرای طبیعی و ذاتی مثل h9 و pbmc انسانی مولد apobec3g استفاده میشود. به دلیل ناکارآمدی آنها در این نوع بررسیها، نیازمند سلولهای ناپذیرایی بودیم که به طور ثابت و به میزان بالا تولید apobec3g بنماید. لذا از سلولهای 293t برای ایجاد این دسته از سلول...
Proteasomal degradation of APOBEC3G is a critical step for human immunodeficiency virus type 1 (HIV-1) replication. However, the necessity for polyubiquitination of APOBEC3G in this process is still controversial. In this study, we showed that although macaque simian immunodeficiency virus (SIVmac) Vif is more stable than HIV-1 Vif in human cells, SIVmac Vif induces degradation of APBOEC3G as e...
The apolipoprotein B mRNA editing catalytic polypeptide-like (APOBEC) family proteins bind RNA and single-stranded DNA, and create C-to-U base modifications through cytidine deaminase activity. APOBEC3G restricts human immunodeficiency virus 1 (HIV-1) infection by creating hypermutations in proviral DNA, while HIV-1-encoded vif protein antagonizes such restriction by targeting APOBEC3G for degr...
APOBEC3 proteins catalyze deamination of cytidines in single-stranded DNA (ssDNA), providing innate protection against retroviral replication by inducing deleterious dC > dU hypermutation of replication intermediates. APOBEC3G expression is induced in mitogen-activated lymphocytes; however, no physiologic role related to lymphoid cell proliferation has yet to be determined. Moreover, whether AP...
It is well established that the cytosine deaminase APOBEC3G can restrict HIV-1 virions in the absence of the virion infectivity factor (Vif) by inducing genome mutagenesis through deamination of cytosine to uracil in single-stranded HIV-1 (-)DNA. However, whether APOBEC3G is able to restrict HIV-1 using a deamination-independent mode remains an open question. In this report we use in vitro prim...
The interplay between the innate immune system restriction factor APOBEC3G and the HIV protein Vif is a key host-retrovirus interaction. APOBEC3G can counteract HIV infection in at least two ways: by inducing lethal mutations on the viral cDNA; and by blocking steps in reverse transcription and viral integration into the host genome. HIV-Vif blocks these antiviral functions of APOBEC3G by imped...
APOBEC3G is a cytidine deaminase with two homologous domains and restricts retroelements and HIV-1. APOBEC3G deaminates single-stranded DNAs via its C-terminal domain, whereas the N-terminal domain is considered non-catalytic. Although APOBEC3G is known to bind RNAs, APOBEC3G-mediated RNA editing has not been observed. We recently discovered RNA editing by the single-domain enzyme APOBEC3A in i...
BACKGROUND APOBEC3G (apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G) has antiretroviral activity associated with the hypermutation of viral DNA through cytosine deamination. APOBEC3G has two cytosine deaminase (CDA) domains; the catalytically inactive amino-terminal domain of APOBEC3G (N-CDA) carries the Vif interaction domain. There is no 3-D structure of APOBEC3G solved b...
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