background:  aeromonas spp. can cause diarrhea and various infections in humans. access to rapid techniques with a high sensitivity and specificity is strongly needed for the identification of aeromonas species. the aim of this study was to evaluate two different methods including api 20e bacterial identification tests and the molecular detection using pcr primers specific for 16s-rrna and 23s-...

background : cockroaches mechanically spread pathogenic agents, however, little is known about their gut microbiota. identification of midgut microbial community helps targeting novel biological control strategies such as paratransgenesis. here the bacterial microbiota of periplaneta americana midgut, were identified and evaluated for finding proper paratransgenesis candidate. methods : midgut ...

Phenotypic identification of gram-negative bacteria from Cystic Fibrosis (CF) patients carries a high risk of misidentification. Therefore, we compared the results of biochemical identification by API 20NE with 16S rRNA gene sequencing in 88 gram-negative, oxidase-positive rods, other than morphologically and biochemically typical P. aeruginosa, from respiratory secretions of CF patients. The A...

BACKGROUND Cockroaches mechanically spread pathogenic agents, however, little is known about their gut microbiota. Identification of midgut microbial community helps targeting novel biological control strategies such as paratransgenesis. Here the bacterial microbiota of Periplaneta americana midgut, were identified and evaluated for finding proper paratransgenesis candidate. METHODS Midgut of...

Six commercially available bacterial identification products were tested with Vibrio alginolyticus (12 strains), V. cholerae (30 strains), Photobacterium (Vibrio) damselae (10 strains), V. fluvialis (10 strains), V. furnissii (4 strains), V. hollisae (10 strains), V. metschnikovii (9 strains), V. mimicus (10 strains), V. parahaemolyticus (30 strains), and V. vulnificus (10 strains) to determine...

The purpose of this study was to test the applicability of the API 20E and API Rapid NFT systems for the identification of some predominant gram-negative and gram-positive bacteria isolated from lab-scale activated sludge treatment systems. In this study, one lab-scale sequencing batch reactor (SBR) and one lab-scale continuous-flow stirred tank reactor (CFSTR) were setup. After both reactors h...

A total of 503 veterinary enteric bacterial pathogens obtained from state veterinary diagnostic laboratories were tested on API 20E strips to determine whether this rapid microidentification system could be utilized for veterinary clinical microbiology. The API 20E strip accurately identified 96% of the veterinary isolates and misidentified 3%. Identifications by the API system and the diagnost...

Nine of 10 strains of Actinobaculum schaalii caused urinary tract infections in predisposed individuals. Identification included 16S rRNA gene sequence analysis and use of the API Coryne and Rapid ID 32 A test systems. A. schaalii is easily overlooked due to its slow growth in ambient air and its resemblance to the normal bacterial flora on skin and mucosa.

Cystic fibrosis (CF) predisposes patients to bacterial colonization and infection of the lower airways. Several species belonging to the genus Burkholderia are potential CF-related pathogens, but microbiological identification may be complicated. This situation is not in the least due to the poorly defined taxonomic status of these bacteria, and further validation of the available diagnostic as...